艾灸胃经穴治疗慢性萎缩性胃炎大鼠的代谢组学研究

Abstract

目的：研究艾灸干预胃经穴对慢性萎缩性胃炎（CAG）大鼠血清、胃、延髓和大脑皮质的影响，探讨艾灸胃经穴促进慢性萎缩性胃炎大鼠胃黏膜损伤修复的病理学变化和相关分子机制及代谢轮廓变化等。 方法：24只清洁级SD大鼠按随机数字表法随机分为4组，即正常组、模型组、艾灸胃经穴组、艾灸非穴位组，每组各6只。除正常组外，其余三组利用每日交替自由饮用氨水溶液（0.1％）和脱氧胆酸钠溶液（20mmol/L）结合饥饿障碍法制备大鼠慢性萎缩性胃炎模型，然后用艾灸分别刺激艾灸胃经穴组大鼠和艾灸非穴位组大鼠相关部位。收集大鼠的血清、胃、延髓和大脑皮质，采用智能生物显微镜下观察各组大鼠胃黏膜病理学变化、酶联免疫吸附测定法...

Objective:Through (1H NMR)、enzyme linked immunosorbent assay (ELISA)、Western blotting (Western Blot WB)and microscopic observation of histopathology were performed by means of nuclear magnetic resonance spectroscopy (1H NMR).Effect of moxibustion and stomach meridian on serum, urine and gastric tissue in rats with chronic atrophic gastritis, and to explore the metabolic and related molecular mecha...