基于实时荧光定量PCR技术的早期梅毒活动性和疗效评价
Evaluation of Therapeutic Efficacy and Disease Activity in Early Syphilis Based on Real-time Quantitative PCR
Abstract
背景：至今，梅毒活动性和疗效评价依然困难，缺乏梅毒疗效评判标准，临床上通常采用血清非特异性抗体滴度和/或滴度四倍下降作为参考指标。然而，有关梅毒患者在梅毒螺旋体（Treponemapallidum，T.pallidum）自然感染过程中非特异性抗体变化仍然不清。本研究建立T.pallidum感染早期梅毒兔模型，监测自然感染和抗生素治疗过程中血清RapidPlasmaReagin(RPR)滴度和皮损中核酸载量变化，以及不同脏器中梅毒螺旋体核酸的分布，并进一步采用脏器标本接种新西兰兔观察感染率和疗效，探究实时荧光定量PCR技术在早期梅毒活动性和疗效判断中的应用价值。 方法：选择梅毒血清抗体阴性的新... Background: Currently, the assessment of the syphilis activity and treatment are extremely difficult and lack of efficacy evaluation criteria. Therefore, clinicians usually, used serum nontreponemal test antibody titers and its four-fold decline in titers as a judgment index for syphilis activity and treatment efficacy evaluation. However, the dynamic change of the nontreponemal test antibody tite...