睡眠缺乏小鼠通过脂质代谢异常破坏角膜上皮细胞微绒毛的形态诱发干眼
Dry Eye Induced by Destroying the Morphology of Corneal Epithelial Cell Microvilli through Abnormal Lipid Metabolism in Sleep Deficient Mice
Date
2018-04-12Collections
- 医学院－学位论文 [896]
Abstract
目的：探讨生理状态下睡眠缺乏小鼠诱发干眼的机制。 方法：以水上支架站立法建立睡眠缺乏（SD）小鼠模型，18g—20g雄性C57BL/6J小鼠随机分为正常对照组（Normal组）、5天SD组（SD5D组）、10天SD组（SD10D组）、非诺贝特处理组（Feno组）、0.1%DMSO对照组（DMSO组）。Feno和DMSO组于造模后第6天分别用10μL200μM非诺贝特和0.1%DMSO滴眼，每天3次。5天SD组于第5天，其余组于第10天进行泪液分泌测定、OGD和PAS染色等干眼临床指标的检测，并取角膜组织进行RNA和蛋白的提取、冰冻和石蜡切片及扫描电镜样本的制备。以免疫组化、qRT-PCR和蛋... Objective: To investigate the mechanism of mice model of dry eye induced by sleep deficiency in under physiological conditions. Methods: 18 to 20g adult C57BL/6J mice were used to establish sleep deficiency (SD) model of mice by using sticks over water. Mice were randomly divided into five groups: Normal control group (Normal group), SD 5D group, SD 10D group, fenofibrate treated group (Feno grou...