新鞘氨醇杆菌US6-1 tonB2基因缺失株 的构建与功能研究

Abstract

NovosphingobiumpentaromativoransUS6-1是一株筛选自Ulsan海湾沉积物的革兰氏阴性菌，能高效降解2环至5环的PAHs。链特异转录组测序发现，苯并芘（BaP）压力下共有13个TonB系统相关基因表达水平显著上调，为揭示TonB系统在菌株US6-1应对BaP压力中的作用，我们以TonB系统中的关键基因tonB为研究对象，在基因组序列分析和实时荧光定量PCR分析的基础上通过自杀性质粒同源重组的方法构建了新鞘氨醇杆菌US6-1tonB基因缺失菌株，并对tonB基因缺失株的生物学特性展开了研究，主要研究结果如下： （1）基因组序列的分析结果表明菌株US6-1有4个t...

Novosphingobium pentaromativorans US6-1, a marine bacterium isolated from muddy sediment of Ulsan Bay, Republic of Korea, is a gram-negative bacterium, which can effectively degrade polycyclic aromatic hydrocarbons that have two to five benzene rings. Strand-specific RNA sequencing show that 13 TonB system-related genes significantly upregulated under BaP stress. In order to reveal the response of...