鲍鱼内脏多糖的提取纯化及生物活性研究
Extraction and purification and biological activity studies of polysaccharides from abalone visceral
Abstract
本研究以莆田汇龙食品有限公司提供的皱纹盘鲍内脏为实验材料，采用碱性蛋白酶、中性蛋白酶、酸性蛋白酶、胃蛋白酶、胰蛋白酶和木瓜蛋白酶六种蛋白酶对其进行酶解，以多糖得率为指标，结果显示碱性蛋白酶酶解所得多糖得率最高，为6.66%。由单因素实验和正交实验，确定了碱性蛋白酶提取鲍鱼内脏多糖的最佳工艺条件。将得到的鲍鱼内脏粗多糖进行体外抗氧化活性测定，结果为100%清除羟自由基的鲍鱼内脏粗多糖浓度为9.6mg/mL，还原能力A700，0.485为8.16mg/mL，100%清除ABTS自由基浓度为7.5mg/mL，表明所得到的多糖具有较好的抗氧化活性。采用双酶解法以提高多糖含量，结果显示胃蛋白酶二次酶解所... The polysaccharides was obtained by using Haliotis discus hannai Ino viscera as material, the alkaline protease, neutral protease, vernase, papain, pepsin and trypsin as protease enzymolysis to release the polysaccharide chain. The experiments results showed that when used alkaline protease, the polysaccharide yield was the highest, which was 6.66%. Then by single factor experiment and the orthogo...