NBA1与PHGDH相互作用并促进乳腺癌的生长
NBA1 Interacts with PHGDH and Promotes Breast Cancer Growth
Abstract
NBA1是BRCA1复合体中重要的接头蛋白,对整个BRCA1复合体的构架以及复合体成员蛋白的稳定性十分重要,它参与DNA损伤复合体的组成并且介导DNA的损伤修复。为了探究NBA1新的功能,我们以NBA1为饵进行了蛋白相互作用的筛查,发现PHGDH与NBA1存在着相互作用。PHGDH作为糖酵解通路的旁支——丝氨酸合成通路的第一个分支酶,是下游一系列反应的重要限速因素。此外,在许多乳腺癌的发生发展过程中PHGDH都扮演了重要的角色。 本文的研究中我们通过一系列实验首次发现NBA与PHGDH存在相互作用并且葡萄糖饥饿刺激增强这种相互作用,而糖酵解中间代谢物3-磷酸甘油酸对这两个蛋白的相互作用有直接... NBA1 is an important adaptor protein in breast cancer type 1 susceptibility protein (BRCA1) complex. It is vital to the structure of BRCA1 complex, as well as the protein stability of the other members in this complex. Functionally, it is involved in the assembling of DNA damage repair complex and required for DNA damage repair. To study novel functions of NBA1, we performed protein interaction sc...