对虾白斑综合症病毒（WSSV）结构蛋白VP41B与非结构蛋白ICP11的功能分析及病毒侵染机制的初步研究
The Function Analysis of Structure Protein VP41B and Non-structure Protein ICP11 of White Spot Syndrome Virus and the Preliminary Research on the mechanism of virus invasion
Date
2018-06-11Collections
- 生命科学－学位论文 [2445]
Abstract
对虾白斑综合症病毒（Whitespotsyndromevirus，WSSV）是一种无包涵体、具双层囊膜、杆状型的双链环状DNA病毒，隶属于线形病毒科(Nimaviridae)白斑病毒属（Whispovirus）。本文围绕着病毒致病相关基因开展研究，包括病毒结构蛋白VP41B和非结构蛋白ICP11的功能进行了分析。此外，本论文还对病毒侵染宿主血细胞的机制进行了初步的研究。 本实验室早先已发现VP41B是病毒囊膜上的蛋白。为了确证其结果，本文用Westernblotting结合免疫电镜观察，证实VP41B确实定位在囊膜上的蛋白，然后利用下拉实验和Far-Westernblotting的方法发现V... White spot syndrome virus (WSSV) is an non-occluded, rod-shaped, enveloped and double-stranded DNA virus. It has been classified as the sole member in a novel virus genus, Whispovirus, of the family Nimaviridae.In this paper, we focused on the viral disease-related genes, including function analysis of structure protein VP41B and non-structure protein ICP11 of WSSV. In addition, we also make preli...