Effects of Elongated Needle with Paired Needling “Shuidao”(ST28) and “Zhibian”(BL54) on Neurological Function of Spinal Cord Injury Rats
- 医学院－已发表论文 
目的观察芒针偶刺秩边与水道穴对大鼠脊髓损伤后神经功能的影响及可能作用机制。方法 30只SD大鼠随机分为假手术组、模型组、芒针治疗组各10只。模型组、芒针治疗组采用改良Allen's法制作脊髓损伤模型。芒针治疗组大鼠手术苏醒后即刻给予芒针秩边和水道穴,用偶刺法针刺大约2 cm深度,捻转1 min,并使同侧秩边、水道组成一回路连接于电针仪上,电针30 min,每日1次,假手术组和模型组正常饲养。5日后采用BBB评分法观察各组大鼠后肢运动功能情况,取脊髓组织行组织学观察、计算细胞凋亡指数、检测Bcl-2、Bax表达情况。结果与假手术组比较,模型组大鼠BBB运动功能评分明显降低,神经细胞凋亡指数升高,脊髓组织Bax、Bcl-2阳性细胞百分率升高(P<0.05或P<0.01)。芒针治疗组大鼠BBB运动功能评分较模型组明显升高、神经细胞凋亡指数降低(P<0.05或P<0.01);芒针治疗组脊髓组织Bax阳性细胞百分率显著低于模型组,Bcl-2阳性细胞百分率显著高于模型组(P<0.01)。结论芒针偶刺秩边与水道穴可能通过调控凋亡相关基因Bax、Bcl-2的表达,减少神经细胞凋亡,从而促进神经功能恢复。Objective To observe effects and possible mechanism of elongated needle with paired needling"Shuidao"( ST28) and"Zhibian"( BL54) on neurological function of spinal cord injury rats. Methods Thirty SD rats were randomly divided into control group,model group and treatment group,10 rats in each group. The model group and the treatment group were used improved Allen 's method to make models of spinal cord injury. In the treatment group,when rats revived after operation,the researchers immediately used elongated needle to needle"Shuidao"( ST28) and "Zhibian"( BL54) with method of paired needling to acupuncture the rats,about 2 cm depth and twirl for 1 min and made two ipsilateral acupoints to form a loop connected to electric acupuncture apparatus,electric acupuncture for 30 min,once per day. After 5 days the researchers used BBB scoring method to observe motor function of rats' hind limbs and took tissue of spinal cord to make histological observation,count apoptotic index and detect expression of the Bcl-2 and Bax. Results Compared with the control group,in the model group,BBB motor function score significantly decreased but nerve cell apoptotic index and percentage of positive cells about Bax and Bcl-2 of spinal cord tissue increased( P < 0. 05 or P < 0. 01). Compared with the model group,in the treatment group,BBB motor function score significantly increased but nerve cell apoptotic index decreased( P < 0. 05 or P < 0. 01); And percentage of positive cells about Bax of spinal cord tissue significantly decreased but percentage of positive cells about Bcl-2 significantly increased( P < 0. 01). Conclusion Elongated needle with paired needle"Shuidao"( ST28) and"Zhibian"( BL54) may promote neural functional to recover by regulating the expression of apoptotic gene that Bax and Bcl-2 to reduce apoptosis of nerve cells.