黄连素对多囊卵巢综合征大鼠高雄激素状态及StAR蛋白表达的影响

Abstract

目的:研究黄连素对多囊卵巢综合征(PCOS)大鼠治疗作用及机制。方法:将48只6周龄SPF级雌性SD大鼠随机分为空白对照组和造模组,造模组采用来; 曲唑(1mg·kg~(-1)·d~(-1))灌胃诱导PCOS模型。造模结束后,将造模成功的大鼠随机分为模型对照组、二甲双胍组、黄连素组,每组11; 只。治疗期间空白对照组、模型对照组均用1%羧甲基纤维素灌胃,二甲双胍组用1%羧甲基纤维素+二甲双胍(135mg·kg~(-1)·d~(-1))灌; 胃,黄连素组用1%羧甲基纤维素+黄连素(81mg·kg~(-1)·d~(-1))灌胃。测定大鼠血清睾酮、空腹胰岛素,光镜下观察大鼠卵巢HE染色病; 理片和StAR免疫组化病理片。结果:本实验模型大鼠出现典型的PCOS表现:卵巢呈多囊样改变;血清睾酮升高(P<0.05);空腹胰岛素升高(P<0; .01),提示存在胰岛素抵抗。二甲双胍和黄连素均能改善模型大鼠的胰岛素抵抗,但黄连素降低睾酮的能力优于二甲双胍(P<0.05)。卵泡膜细胞的细胞; 膜上StAR密度随睾酮值升高而升高。结论:黄连素有治疗PCOS的作用,它不仅能改善胰岛素抵抗,还能通过降低卵泡膜细胞的细胞膜上StAR密度降低血; 清睾酮值,治疗高雄激素血症。

Objective: To study the therapeutic effect and mechanism of berberine on; rats with polycystic ovarian syndrome. Methods: Forty-eight six-week SPF; female SD rats were randomly divided into blank control group and model; group. The model group received letrozole to induce PCOS model, then the; successful modeling rats undergone were divided into model control; group, melbine group and berberine group. During the treatment, 1%; carboxymethylcellulose was used on the blank control group and model; control group, 1% carboxymethylcellulose plus melbine were used on the; melbine group while 1% carboxymethylcellulose plus berberine were used; on the berberine group. The testosterone and fasting insulin of rats; were detected, the ovarian pathological slices of HE staining and the; StAR immunohistochemical pathological slices of rats were observed under; light microscopes. Results: In this experiment, the model rats have; typical PCOS manifestations: the ovary has polycystic changes; the; testosterone rises (P<0.05); the fasting insulin rises (P<0.01), which; indicate that insulin resistance exists. Both melbine and berberine can; improve the insulin resistance of model rats, but the performance of; berberine in lowering androgen is better than melbine. The testosterone; of PCOS hyperandrogenism is positively related to the StAR density on; the cytomembrane of theca cells. Conclusion: Berberine has effect on; PCOS. It can not only improve insulin resistance but also treat; hyperandrogenism by lowering the StAR density on the cytomembrane of; theca cells to decrease the testosterone.