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dc.contributor.author王丽惠
dc.contributor.author程变巧
dc.contributor.author朱琪
dc.contributor.author林伟国
dc.date.accessioned2018-11-26T08:58:48Z
dc.date.available2018-11-26T08:58:48Z
dc.date.issued2017
dc.identifier.citation基础医学与临床,2017,(9)
dc.identifier.issn1001-6325
dc.identifier.other10.16352/j.issn.1001-6325.2017.09.010
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/166172
dc.description.abstract目的探讨转化生长因子-β1(TGF-β1)在大鼠肝星状细胞系(HSC-T6)活化及上皮间质转换(EMT)中的作用。方法体外培养HSC-T6,用MTT法筛选TGF-β1对HSC-T6作用的最佳浓度;用10μg/L TGF-β1处理HSC-T624h,相差倒置显微镜下观察细胞形态改变,免疫荧光染色法检测细胞骨架结构F-actin蛋白的表达,RT-qPCR法检测肌动蛋白α-SMA及代表上皮间质转换的神经黏附素(N-cadherin)、波形蛋白(vimentin)和上皮黏附素(E-cadherin)基因表达;用不同浓度(0、5和10μg/L)的TGF-β1处理HSC-T624h,Western blot检测α-SMA、N-cadherin、vimentin和E-cadherin蛋白表达。结果10μg/L TGF-β1干预HSC-T624h有最好的细胞存活率;TGF-β1刺激HSC-T6后,细胞拉伸,伪足增多呈星形,细胞间连接疏松,呈显著活化状态;F-actin聚集形成应力纤维丝,沿细胞长轴分布;实验组α-SMA mRNA及vimentin mRNA的表达量明显高于对照组(P〈0.05),而E-cadherin mRNA的表达量明显降低(P〈0.05);在不同浓度的TGF-β1呈剂量依赖性致α-SMA及N-cadherin和vimentin的蛋白表达量增多,而E-cadherin的蛋白表达量减少。结论TGF-β1可诱导HSC-T6活化及上皮间质转换。
dc.description.abstractObjective To observe the effect of TGF-β1 on activation and epithelial mesenchymal transition (EMT) in rat hepatic stellate cell-T6. Methods Adopt the MTT method to screen the optimum concentration of TGF-β1 in vitro HSC-T6 cultured. After the HSC-T6 stimulation by TGF-β1 of 10 μg/L for 24 hours, the morphology of the cells was observed under inverted phase contrast microscope, the expression of F-actin which on behalf of cotoskeletal structure was detected by immunofluorescence staining; the expression of α-SMA and N-cadherin, vimentin, E-eadherin was measured by RT-qPCR ; The changes of α-SMA, N-cadherin, vimentin and E-eadherin were assessed by Western blot after different concentrations (0,5 and 10 μg/L) of TGF-β1 interventing HSC-T6 for 24 h. Results The optimal cell survival rate was recorded when 10 μg/L TGF-β1 dealt with cells for 24 h. After HSC-T6 were treated with TGF-β1 ,cells stretched, pseudopodia increased and turn into stellate, ceils connections were looser, so that represented a significantly activated state. F-actin filaments gathered to form stress and distributed along the long axis of the cells ; The expression of α-SMA mRNA and vimentin mRNA in experimental group was significantly higher while E-cadherin mRNA was obviously lower than the control group (P〈 0. 05). TGF-β1 made the protein expression of α-SMA and N-cadherin, vimentin in dose-dependent increased while E-cadherin was decreased.Conclusions TGF-β1 may induce activation and epithelial-mesenchymal transition of HSC-T6.
dc.description.sponsorship福建省科技计划(2015Y01010224);福州市科技计划(2014-S-137-1)
dc.language.isozh_CN
dc.subjectTGF-Β1
dc.subject肝星状细胞
dc.subject活化
dc.subject上皮间质转换
dc.subjectepithelial-mesenchymal transition
dc.titleTGF-β1诱导大鼠肝星状细胞系HSC-T6活化及上皮间质转换
dc.title.alternativeTGF-β1 induces activation of HSC-T6 cells and epithelial-mesenchymal transition in rats
dc.typeArticle


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