Effects of Shenling Baizhu Powder on expression of apoptosis related proteins of tumor chemotherapy model mice with H22 hepatocellular carcinoma cells
- 医学院－已发表论文 
目的:研究参苓白术散(SLBZP)对H22肝癌移植瘤小鼠化疗后肿瘤凋亡相关因子Caspase -3、; Caspase-9、X染色体连锁凋亡抑制因子(XIAP)、血小板源生长因子-BB(PDGF-BB)、血管生成素-1(Ang-1)、血管生成素-2; (Ang-2)表达的影响。方法:SPF级昆明小鼠64只,将肝癌H22细胞悬液(2*10~7/mL)接种于小鼠右前腋皮下,1周全部形成移植瘤后,行; 环磷酰胺(CTX,0.20g/kg,0.2mL/10g)腹腔注射,建立肿瘤化疗模型。随机分组为8组:模型组,CTX(0.02g/kg)组,SLB; ZP高、中、低(6.00、3.00、1.50g/kg)剂量组,SLBZP高、中、低剂量+CTX(0.02g/kg)组,给药14d后,运用蛋白印迹; 法(Western blot)测定小鼠瘤组织中Caspase-3、; Caspase-9、XIAP蛋白表达;酶联免疫吸附(ELISA)测定小鼠血清PDGF-BB、Ang-1、Ang-2表达。结果:SLBZP联合治疗; 各组抑瘤作用明显,CT X组,SLBZP高剂量组,SLBZP高、中剂量+C T X组抑瘤率分别为52.39%、; 45.84%、58.41%、52.77%。与模型组比较,各治疗组血清PDGF-BB、Ang-1、Ang-2及瘤组织中XIAP表达呈现出被下调的趋; 势,瘤组织中Caspase-3、Caspase-9表达呈现出被上调的趋势,且以SLBZP高剂量加CTX组变化幅度最为显著(P<0.01,P<0.; 05)。结论:SLBZP联合化疗可以更有效调节H22肝癌移植瘤小鼠肿瘤凋亡相关因子的表达,促进肿瘤细胞凋亡可能是其改善化疗的机制之一。Objective: To investigate the effects of Shenling Baizhu Powder (SLBZP); on expression of apoptosis related proteins such as Caspase-3,; Caspase-9, X-linked inhibitor of apoptosis (XIAP), PDGF-BB, Ang-1, Ang-2; of tumor chemotherapy model mice with H22 hepatocellular carcinoma; cells. Methods: Sixty-four Kunming mice were subcutaneously injected; with suspension of H22 hepatocellular carcinoma cells (2*10~7/mL) into; the right anterior armpit. After 1 week, all transplanted tumors were; formed and the mice were received intraperitoneal injection with cytoxan; (CTX) with the dosage of 0.2g/kg to establish the tumor chemotherapy; model. Then mice were randomly divided into eight groups. CTX group was; treated with CTX (0.02g/kg), the model group was treated with; physiologic saline, three SLBZP groups were treated with SLBZP (6.00,; 3.00, 1.50g/kg), and other three groups were treated with SLBZP (6.00,; 3.00, 1.50g/kg) plus CTX (0.02g/kg). After all groups were treated for; 14 days, Western blot was used to detect the expression of Caspase-3,; Caspase-9 and XIAP proteins in tumor tissue, and protein expression of; PDGF-BB, Ang-1, Ang-2 in blood was measured by enzyme-linked; immunosorbent assay (ELISA). Results: The combined treatment group had; obvious effect on tumor inhibition. Tumor inhibitory ratios in CTX; alone, SLBZP (H), SLBZP (H) plus CTX, and SLBZP (M) plus CTX groups were; 52.39%, 45.84%, 58.41% and 52.77% respectively. Compared to the model; group, the protein expressions of PDGF-BB, Ang-1, Ang-2, and XIAP in; tumor tissue were obviously lower than those in all treated groups. And; the expressions of Caspase-3 and Caspase-9 in tumor tissue showed a; trend of up-regulation. And the effect in SLBZP (H) plus CTX group was; the most significant (P<0.01, P<0.05). Conclusion: SLBZP can more; effectively adjust the expression of apoptosis related proteins of tumor; in mice with H22 hepatocellular carcinoma cells, and promoting tumor; apoptosis may be one of the possible mechanisms of SLBZP to improve; chemotherapy in treating hepatic cancer.