中药组分HJJB方对高脂饮食诱导的非酒精性脂肪性肝炎大鼠的防治作用

Abstract

目的:探讨中药组分HJJB方(红景天苷、姜黄素、绞股蓝总苷、白术多糖)对高脂饮食诱导的大鼠非酒精性脂肪性肝炎的防治作用。方法:采用高脂饮食14周; 诱导大鼠非酒精性脂肪性肝炎模型。在造模第9周起,随机分为模型组,HJJB方高、低剂量组,罗格列酮组,灌胃给药6周。观察肝组织病理变化(HE染色); ,肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酰转肽酶(GGT)活性及TG、总胆; 固醇(TC)含量的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA含量较正常组显著升高(P<0.01),血清ALT; 、AST、GGT活性及TG、TC含量较正常组亦明显升高(P<0.01)。HJJB方高、低剂量组的上述病理改变显著减轻,肝组织TG、FFA含量及血; 清ALT、AST、 GGT、TG、TC水平显著低于模型组(P<0.01),其中HJJB方高剂量组的肝组织TG、FFA含量和血清ALT、AST、; GGT活性显著低于HJJB方低剂量组和罗格列酮组(P<0.05,P<0.01)。结论:中药组分HJJB方对高脂饮食诱导的大鼠非酒精性脂肪性肝炎具; 有良好的防治作用。

Objective: To discuss the prevention and treatment of HJJB (salidroside,; curcumin, gypenoside and atractylodes macrocephala polysaccharide); compound on non-alcoholic steatohepatitisin rats induced by high-fat; diet. Methods: Non-alcoholic steatohepatitis model was induced by; high-fat diet for 14 weeks. From the ninth week, the rats were randomly; divided into model group, HJJB high-dose group, HJJB low-dose group and; rosiglitazone group, and were given gavage for six weeks. The observing; items including: pathological changes of liver tissue (HE staining); the; changesof contents of liver tissue triglyceride (TG) and free fatty acid; (FFA); the activities of serum alanine aminotransferase (ALT), aspartate; aminotransferase (AST), glutamyl transpeptidase (GGT) and contents of; serum total cholesterol (TC) and TG. Results: Significant hepatocellular; steatosis and vacuolar degeneration were observed inthe liver tissue of; the model group. The contents of TG and FFA in liver tissue of model; group were significantly higher than those of normal group (P<0.01), and; the activities of serum ALT, AST, GGT and the contents of TG and TC of; model group were higher than those of normal group too (P<0.01). Hepatic; pathological changes in the HJJB compound high-dose, low-dose groups; were all significantly meliorated. The levels of liver TG, FFA and serum; ALT, AST, GGT, TG and TC of HJJB high-dose group, HJJB low-dose group; were significantly lower than that of model group (P<0.01). In addition,; the contents of liver TG and FFA and serum ALT, AST, GGT of HJJB; compound high-dose group were lower than those of HJJB compound low-dose; and rosiglitazone group (P<0.05, P<0.01). Conclusion: HJJB compound does; well in the prevention and treatment of non-alcoholic steatohepatitis; induced by high-fat diet.