Effect of Sanren Tang (三仁汤) on Mucin 5AC, Aquaporin 1 and Aquaporin 5 in Lung Tissue of Damp-heat Stressed Mice
- 医学院－已发表论文 
目的探讨三仁汤防治湿热环境下呼吸系统疾病的可能作用机制。方法72只小鼠随机分为空白组、模型组、三仁汤组,每组24只。空白组普通环境喂养,其余两组; 置入气候箱接受高温高湿刺激。三仁汤组小鼠于放入气候箱前、放入气候箱后第12h分别予三仁汤(浓度1g/ml)0.2ml灌胃各1次,空白组和模型组给; 予等量生理盐水灌胃。放入气候箱后第12h、24h、4天时每组取材8只,检测肺组织黏蛋白5AC; (MUC5AC)、水通道蛋白1(AQP1)、水通道蛋白5(AQP5)表达水平。结果模型组第24h时MUC5AC水平高于空白组,三仁汤组第12h; 、24h时MUC5AC表达低于模型组(P<0.05)。三仁汤组第12h、24h时AQP5水平显著高于空白组,第24h时高于模型组(P<0.05); 。各组第4天时MUC5AC、AQP5表达水平比较差异无统计学意义(P>0.05)。各组不同时间点AQP1表达水平比较差异均无统计学意义(P>0.; 05)。结论三仁汤可能通过促进肺组织AQP5表达,从而抑制MUC5AC分泌,阻止湿热环境下呼吸系统疾病气道黏液高分泌状态进展。Objective To explore the possible mechanism of Sanren Tang (三仁汤) in; preventing and treating respiratory diseases under damp-heat; circumstances. Methods Seventy-two mice were randomized into blank; group, model group and Sanren Tang group, with 24 mice in each group.; The blank group was fed under conventional environment, while the other; two groups were put into climate chamber with high temperature and high; humidity. The Sanren Tang group was given Sanren Tang (1g/ml 0.2ml)by; gavage before and at the 12th hour of being put into climate chamber.; The blank group and model group were given equivalent normal saline by; gavage. At the 12th, 24th hour and on the 4th day after being put into; climate chamber, eight mice in each group were selected to draw; materials, and the levels of mucin 5AC (MUC5AC), aquaporin 1 (AQP1) and; aquaporin 5 (AQP5) were detected. Results The 24th-hour MUC5AC level in; the model group was higher than that in the blank group; the MUC5AC; level at the 12th and the 24th hour in Sanren Tang group was; respectively lower than that in the model group (P<0.05). In Sanren Tang; group, the AQP5 level at the 12th and the 24th hour was significantly; higher than that in the blank group, and at the 24th hour, it was higer; than that of the model group (P<0.05). On the 4th day, there was no; significant difference of MUC5AC level and AQP5 level among groups; (P>0.05). In each group, there was no significant difference of AQP1; level at different time points (P>0.05). Conclusion By promoting the; expression of AQP5 in lung tissue, Sanren Tang may inhibit the secretion; of MUC5AC, and prevent the airway mucus hypersecretion in respiratory; diseases under damp-heat circumstances.