Construction of a Neotype Controllability Magnetic Targeting Restructuring Truncated Tissue Factor
- 医学院－已发表论文 
目的:构建新型的具有可控性且能诱发动物血栓的栓塞剂磁靶向凝血蛋白,从而为动物血栓模型的建立提供新工具。方法:使用化学共沉淀法合成磁流体(; magnetic nanoparticles, MNPs),通过透析对其纯化后用超导量子干涉磁强针(; SQUID)测定其磁性能;利用基因工程方法表达截短组织因子( truncated tissue factor,tTF); (又称为凝血蛋白)并纯化与鉴定;采用戊二醛交联法构建磁靶向凝血蛋白(; MNPs-tTF),体外分析其磁控靶向性及凝血效力。结果:成功构建了磁靶向凝血蛋白MNPs-tTF,体外实验证实其保留有磁流体MNPs的磁控靶向; 性,以及凝血蛋白tTF的凝血效力。结论:成功构建了磁靶向凝血蛋白MNPs-tTF,为血栓模型动物的建立提供一种新工具。Objective: To establish a neotype controllability magnetic targeting; restructuring truncated tissue factor s for establish animal models of; thrombosis. Methods: Magnetic nanoparticles ( MNPs) were prepared by; chemical coprecipitation,and employed the superconducting quantum; interference device to characterized the MNPs magnetization. The; truncated tissue factor ( tTF) were expressed efficiently in E. coli and; purification by gene engineering technology. MNPs conjugated the tTF by; glutaraldehyde to compose MNPs-tTF. In vitro analysis the effects of; magnetic targeting and blood coagulation of MNPs-tTF. Result: The; magnetic targeting restructuring truncated tissue factor composites were; build successfully. They are reserve efficient coagulation effect and; magnetic targeted ability in vitro. Conclusion: We have successfully; prepared magnetic targeting thrombin system MNPs-tTF have been; successfully prepared,which maybe provide a basis for developing the; establish animal models of thrombosis.