油茶醇提物对2型糖尿病小鼠血糖的作用

Abstract

目的筛选油茶（Camellia oleifera Abel.）降低2型糖尿病小鼠血糖的有效部位。方法采用腹腔注射烟碱、链脲霉素诱导的2型糖尿病小鼠模型,应用超声提取法分别获得油茶叶、果、籽的乙醇提取物,筛选降低血糖的有效部位。结果阳性药罗格列酮每日灌胃剂量10mg/kg·BW,连续灌胃21d后,降低了2型糖尿病小鼠血糖。分别灌胃油茶叶、油茶果、油茶籽乙醇提取物,按每日剂量100mg/kg·BW连续灌胃21d后,均在一定程度上降低2型糖尿病小鼠血糖,油茶籽乙醇提取物对口服葡萄糖耐受试验（Oral glucose tolerance test,OGTT）作用最明显。与对照组相比,1μM和2μM的阳性药罗格列酮均能明显促进3T3-L1前脂肪细胞分化;油茶籽乙醇提取物在0.5μg·m L-1和1μg·m L-1浓度下也体现出促进分化的活性。结论油茶籽乙醇提取物降低了2型糖尿病小鼠的血糖,促进了3T3-L1前脂肪细胞的分化,但其具体作用机制尚需深入研究。

OBJECTIVE To investigate effective parts possessing property of lowering blood glucose in type 2diabetic mice from Camellia oleifera Abel. METHODS Leaf,fruit and seed ethanol extract of C. oleifera were obtained through ultrasonic extraction,and were subsequently subject to type 2 diabetic mice model induced by intraperitoneal injection of streptozocin and nicotine. RESULTS In accordance with the positive drug rosiglitazone by intragastric administration once daily dose of 10 mg / kg·BW,continuous intragastric administration for 21 days,blood glucose in type 2 diabetic mice was reduced. In accordance with a daily dose of 100 mg / kg·BW leaf,fruit and seed ethanol extract of C. oleifera,continuous intragastric administration for 21 days respectively,in a certain extent,blood glucose in type 2 diabetic mice was reduced. Moreover,effect of seed ethanol extract on blood glucose and oral glucose tolerance test（ OGTT） is the most obvious. Compared with control group（ treated with insulin）,1μM and 2μM of positive drug rosiglitazone can significantly promote differentiation of 3T3-L1 pre-adipocytes,C. oleifera seed ethanol extract as the most effective part for lowing blood glucose at 0. 5μg·m L~（-1） and 1μg·m L~（-1）concentration also promoted 3T3-L1 pre-adipocyte differentiation. CONCLUSION It was suggested that C. oleifera seed ethanol extract lowered blood glucose in type 2 diabetic mice and enhanced differentiation of 3T3-L1 pre-adipocytes,but the specific mechanism needs further study.