Analysis and Study on the Determination of Serum Concentration of Meth- otrexate b~ HPLC
- 药学院－已发表论文 
目的探讨甲氨堞呤血药浓度采用高效液相色谱法测定临床效果。方法用lO％高氯酸对血浆样品的蛋白沉淀后，取上清液分析，应用高效液相色谱法对血药浓度测定。结果甲氨蝶呤在75～1500ng·mL^-l时，存在较为良好的线性关系，75ng·mL。为最低检测限；不同浓度日内日间平均RSD均〈5％。结论高效液相色谱法测定具快速、灵敏、简单优点，可用于甲氨蝶呤药物动力学研究和血药浓度监测。OBJECTIVE To investigate the clinical effect of high performance liquid chromatography for deter- mination of serum concentration of methotrexate. METHODS The protein of plasma samples was precipitated by 10% perchloric acid, and the supernate was obtained. The plasma concentration was determined by high performance liquid chromatography. RESULTS There was a good linear relationship between 75 - 1500ng · mL ^-1 and 79ng · mL^-1 was the lowest detection limit. The average daily RSD was 〈 5%. CONCLUSION High performance liquid chromatography is rapid, sensitive and simple. It can be used for the pharmacokinetic study and blood drug concentra- tion monitoring of rnethotrexa.