HPLC法测定维生素B族片中3种组分的含量

Abstract

目的 采用高效液相色谱法同时测定维生素B族片中三种维生素B（维生素B1,维生素B2,维生素B6）的含量。方法 采用C18柱,以0.005mol·L-1的庚烷磺酸钠溶液（含0.5%冰醋酸和0.05%三乙胺）-甲醇（65：35,v：v）为流动相,流速为1.0m L·min-1。紫外检测波长为280nm。结果 本实验中维生素B1,B2,B6线性范围分别为2.964~74.1mg·m L-1,2.934~73.35mg·m L-1,2.996~74.9mg·m L-1,回收率分别为97.5%,99.7%,100.5%,RSD分别为1.21%,1.5%,0.9%。结论 本法快速、简便、分离效果好,适用于多维片中维生素B1,维生素B2,维生素B6的质量控制。

OBJECTIVE To develop an HPLC method for the determination of three vitamin B in compound vi- tamin tablets. METHODS HPLC, C18 column was used, a mixture of 0. 005mol . L-1 sodium heptanesulfonate （containing 0. 5% glacial acid and 0. 05% triethylamine）-methanol（72：28 ,v/v）as mobile phase at a flow rate of 1.0mL . min-1. The UV detection wavelength was 280nm. RESULTS The calibration curves Showed good linearity in the range of 2. 964 -74. 1mg. mL-1,2. 934 -73.35mg. mL-1 ,2. 996 -74. 9mg . mL-1 ,recovery rate of vita- mins were 97. 5% ,99. 7%, 100. 5%, and RSDs were 1.15% ,0. 77% ,0. 80% respectively. CONCLUSION The method is rapid, convenient and reliable and could be used for quality control of compound vitamin B tablets.