Screening research on effective parts of Alisma orientalis( Samuel. ) Juzep. associated with blood glucose decrease in type 2 diabetic mice
- 药学院－已发表论文 
目的初步探索泽泻[Alisma orientalis(Samuel.)Juzep.]降低2型糖尿病小鼠血糖的有效部位。方法采用超声提取法获得泽泻乙醇提取物,再分别用石油醚、乙酸乙酯、水饱和正丁醇和水相进行萃取分离,得到的泽泻乙醇提取物及其各溶剂萃取物应用于腹腔注射烟碱、链脲霉素诱导的2型糖尿病小鼠模型。造模成功后,将动物分组,其中空白对照组(未注射链脲霉素和烟碱的小鼠)和2型糖尿病模型组小鼠连续灌胃0.9%Na Cl溶液21 d(灌胃剂量为10μl/g),测试药物组分别连续灌胃泽泻乙醇提取物、泽泻石油醚萃取物、泽泻乙酸乙酯萃取物、泽泻正丁醇萃取物、泽泻水萃取物21 d(各测试药物灌胃高、中、低剂量分别为100μg/g、50μg/g、25μg/g),阳性对照组连续灌胃罗格列酮溶液21 d(灌胃剂量为10μg/g),每组8只小鼠,筛选具有降低2型糖尿病小鼠血糖的泽泻有效部位。用具有降低2型糖尿病小鼠血糖的有效部位处理3T3-L1前脂肪细胞,观察其对前脂肪细胞分化过程的影响。结果与2型糖尿病模型组比较,连续灌胃泽泻乙酸乙酯萃取物21 d(剂量为100μg/g)降低了2型糖尿病小鼠的血糖,改善了口服葡萄糖耐受试验(oral glucose tolerance test,OGTT)过程中的糖耐量(P<0.01)。1 mg/L和10 mg/L的泽泻乙酸乙酯萃取层均能促进前脂肪细胞的分化过程。结论泽泻乙酸乙酯萃取物降低了2型糖尿病小鼠血糖,促进了3T3-L1前脂肪细胞分化,但其具体作用机制尚需深入研究。Objective To explore the effective parts of Alisma orientalis( Samuel.) Juzep. associated with blood glucose decrease in type 2 diabetic mice.Methods Ethanol extracts from A. orientalis were obtained by ultrasonic extraction. Then,the ethanol extract was extracted and separated with petroleum ether,ethyl acetate,water saturated n-butanol and water respectively. After decompressing concentration,these solvent extractions were applied to type 2 diabetic mice model induced by intraperitoneal injection of streptozotocin and nicotine. After successful modeling,the mice were divided into the blank control group( without injection of streptozotocin and nicotine),model group of type 2 diabetes mellitus,positive control group and test drug groups,8 in each group. The blank control group and model group were treated with 0. 9% Na Cl at the dose of 10μl / g,the test drug groups were treated with corresponding extracts from different solvent extractions described above at the high-,mid-,low-dose of 100,50,25 μg / g respectively. The positive control group was treated with rosiglitazone at the dose of10μg / g. The treatment course was 21 days for intragastric administration. The effective parts of Alisma orientalis( Samuel.) Juzep. associated with blood glucose decrease in type 2 diabetic mice were screened and then the effect of the screened effective part on the differentiation of 3T3-L1 pre-adipocyte was observed.Results Compared with the model group,the blood glucose was decreased after the intragastric administration with ethyl acetate extract of A. orientalis at dose of100μg / g for 21 days and the glucose tolerance during the oral glucose tolerance test( OGTT) was improved( P < 0. 01). The differentiation of pre-adipocyte was promoted when the ethyl acetate extract of A. orientalis at the concentration of 1 mg / L and 10 mg / L. Conclusion The ethyl acetate extract of A. orientalis reduced the blood glucose in type 2 diabetic mice and promoted the differentiation of 3T3-L1 pre-adipocytes,but the specific mechanism still need to be further studied.