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dc.contributor.authorMing Yangzh_CN
dc.contributor.author杨明zh_CN
dc.contributor.authorKe-Jian Wangzh_CN
dc.contributor.author王克坚zh_CN
dc.contributor.authorJun-Hui Chenzh_CN
dc.contributor.author陈君慧zh_CN
dc.contributor.authorHai-Dong Quzh_CN
dc.contributor.author曲海东zh_CN
dc.contributor.authorShao-Jing Lizh_CN
dc.contributor.author李少菁zh_CN
dc.date.accessioned2011-04-26T08:02:06Z
dc.date.available2011-04-26T08:02:06Z
dc.date.issued2007zh_CN
dc.identifier.citationFish & Shellfish Immunology,2007(5):1060-1071zh_CN
dc.identifier.otherdoi:10.1016/j.fsi.2007.04.011zh_CN
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/1656
dc.description.abstract【英文摘要】Hepcidin is an antimicrobial peptide and putative iron regulatory hormone previously described in mice and humans. Dozens of fish hepcidins have been isolated and characterized so far. Here we present seven hepcidin-like cDNA sequences named AS-hepc1–7, amplified from the normal commercially cultured fish (black porgy) by RACE–PCR. Sequence analysis reveals that these seven potential hepcidin peptides have highly conserved sequences with other known hepcidins, but they are different from each other in constitution and characteristics of predicted mature amino acids. Based on the study, it is deduced that AS-hepc1–7 represent different variants of a family of hepcidin genes in black porgy. To understand the organization of these hepcidin-like genes, we sequenced AS-hepc2 DNA, AS-hepc3 DNA, AS-hepc4 DNA, AS-hepc7 DNA and AS-hepc2 upstream region; and all of the four genomic DNAs consisted of two introns and three exons, the same organization as other reported hepcidins. The tissue-specific gene expression of hepcidins in normal black porgy was evaluated using RT–PCR and dot blot approaches. RT–PCR showed that transcripts of hepcidin-like mRNAs were present in each tested tissue of normal juvenile black porgy, including liver, spleen, kidney, heart, brain, stomach, intestine, gill, skin and blood, but abundant hepcidin-like mRNA transcripts were only detected in the liver, kidney, spleen, intestine and stomach by dot blot assay. In addition, using dot blot and Northern blot approach, a significant increase of hepcidin mRNA transcription was observed in the liver within 48 h after immersion in a suspension of live bacteria, which suggested that the expression pattern of hepcidin-like genes in black porgy might be different in the liver from the other tissues as previously reported in several hepcidin studies.zh_CN
dc.language.isoenzh_CN
dc.publisher《Fish & Shellfish Immunology》编辑部zh_CN
dc.subjectAcanthopagrus schlegeliizh_CN
dc.subjectBlack porgyzh_CN
dc.subjectHepcidinzh_CN
dc.subjectGenomic organizationzh_CN
dc.subjectGene expressionzh_CN
dc.titleGenomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)zh_CN
dc.typeArticlezh_CN


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