Study on the central response mechanism in gastric ulcer rats treated by electroacupuncture using ~1H NMR metabolomics techniques
- 物理技术－已发表论文 
目的:采用核磁共振氢谱技术(~1H; NMR)研究电针胃经穴治疗应激性胃溃疡大鼠大脑皮质、下丘脑、延髓代谢物谱的表达。方法:40只Wistar大鼠随机分为正常组、模型组、胃经组和胆经; 组。利用束缚冷应激法制备应激性胃溃疡模型大鼠,造模成功后用电针分别刺激胃经组和胆经组相关穴位。收集大鼠大脑皮质、下丘脑、延髓,获取一维~1H; NMR谱,并利用模式识别方法分析各组间的代谢轮廓差异。结果:模型组与正常组、胃经组、胆经组大鼠大脑皮质、下丘脑和延髓代谢物谱均有明显区分,其中正; 常组与模型组分离最明显,胃经组最接近正常组,说明其代谢物谱趋向正常。与模型组比较,胃经组大鼠大脑皮质乳酸、萘乙酸、甘露醇等表达上升(P<0.05; ),而肌酸、腺苷、肌苷等表达下降(P<0.05),下丘脑乳酸、肌醇、谷氨酸酯等表达上升(P<0.05),gamma-氨基丁酸等表达下降(P<0.; 05),延髓乳酸、谷氨酸脂、肌醇、丙氨酸等表达上升(P<0.05),而萘乙酸、肌酸等表达下降(P<0.05)。结论:电针胃经穴可调节胃溃疡大鼠大; 脑皮质、下丘脑、延髓的代谢产物表达,大脑皮质、下丘脑和延髓是电针胃经穴治疗胃溃疡的中枢响应位点。Objective: To study the metabolic profiling of the cerebral cortex,; hypothalamus and medulla oblongata from irritable gastric ulcer rats; treated with electroacupuncture on acupoints of the stomach meridian; with 1H nuclear magnetic resonance (~1H NMR) spectroscopy. Methods:; Forty SD rats are randomly divided into the control group, the model; group, the stomach meridian group and the gallbladder meridian group.; The irritable gastric ulcer rat model was established by water-immersion; and restraint stress method. After modeling, the rats from the stomach; meridian group and the gallbladder meridian group were treated with; electroacupuncture on the stomach meridian and gallbladder meridian; respectively. ~1H NMR spectroscopy was applied to detect the metabolic; profiling of the cerebral cortex, hypothalamus and medulla oblongata,; then analyzed metabolic contour differences among the groups using; pattern recognition method. Results: Obvious distinctions were found; between the metabolites spectrum of the cerebral cortex, hypothalamus; and medulla oblongata from the model group, the normal group, the; stomach meridian group and the gallbladder meridian group. More; significant differences were found between the normal group and the; model group, while the metabolites spectrum of the stomach meridian; group was more distinctly close to that in the normal group, indicating; that metabolites spectrum of the stomach meridian group was likely to; normal. Compared with the model group, the concentration of lactic acid,; acetic acid, mannitol increased while creatine, adenosine and inosine; decreased in cerebral cortex of the stomach meridian group (P<0.05), the; concentration of lactic acid, glutamic acid, inositol and alanine; increased while gamma aminobutyric acid decreased in hypothalamus of the; stomach meridian group (P<0.05), the concentration of lactate,; glutamate, myo-lnositol, alanine increased (P<0.05) and naphthylacetic; acid and creatine decreased in medulla oblongata of the stomach meridian; group (P<0.05). Conclusion: Electroacupuncture on acupoints of the; stomach meridian could regulate specific metabolites in the cerebral; cortex, hypothalamus and medulla oblongata, indicating that the cerebral; cortex, hypothalamus and medulla oblongata as the central response site; in the treatment of gastric ulcer by electroacupuncture on acupoints of; the stomach meridian.