Toxic Effects of Four Currently-used Pesticides on Zebrafish Embryonic Development
- 生命科学－已发表论文 
为了研究4种农药(腈苯唑、吡唑醚菌酯、恶霜灵、戊菌隆)单独及联合作用对斑马鱼胚胎发育的影响,设计7个实验组(空白组、溶剂组、4种农药单独暴露组及; 联合暴露组)对斑马鱼胚胎暴露72 h,结果显示,浓度50; ng/L的4种农药单独暴露导致心包囊和卵黄囊水肿比率、脊柱弯曲率均显著高于对照组;腈苯唑、恶霜灵及联合暴露导致斑马鱼仔鱼心率显著下降,出现显著的; 心律不齐;斑马鱼胚胎的Na~+/K~+-ATP酶和Ca~(2+)-ATP酶活性被显著诱导,而联合暴露表现出显著的抑制作用。结果说明,环境浓度的农; 药暴露对鱼类胚胎发育具有明显的毒性。In order to study the effects of four; pesticides(fenbuconazole,pyraclostrobin,metoxazon,and; pencycurone),either individually or jointly,on the development of; zebrafish embryos,seven experiments(blank control,solvent; control,individual of 4 pesticides,and jointly combined four; pesticides)were designed to treat zebrafish embryos for 72 h. The; results showed that individual exposure to any of 4 pesticides at 50; ng/L led to the significant elevation on the rate of pericardial and; yolk sac edema and the frequency of dorsal curvature in the larvae; compared to the control. The exposure to fenbuconazole,metoxazon,and; combined four pesticides resulted in a significant reduction in the; heart rate and a significant increase in cardiac arrhythmia in the; zebrafish larvae. The activities of Na~+/K~+-ATPase and Ca~(2+)-ATPase; of the embryos were significantly induced by the individual exposure to; 4 pesticides,while the both activities were remarkably inhibited by the; combined exposure. The results confirmed that these pesticides at; environmental relevant concentration presented the clear toxicity to the; development of fish embryo.