福建沿海沉积物放线菌的多样性及生物活性

Abstract

目的探索福建沿海沉积物中可培养海洋放线菌的多样性及其抗菌、抗肿瘤活性,为发现新的药物先导化合物提供新菌源。方法从15份福建沿海沉积物选择性分离放; 线菌;16S; rRNA基因序列分析多样性;采用10种活性筛选模型分析生物活性。结果共分离到216株海洋放线菌,MOPS-脯氨酸培养基的分离效果最好。选取77株; 代表菌株进行16S; rRNA基因序列分析,发现其归属于14个属,其中10株为潜在新种。有164株(77%)海洋放线菌对8种指示菌中的一种或几种具有抑制作用;有39株; (18.1%)对HeLa细胞或HepG2细胞有细胞毒活性(抑制率≥50%)。结论福建沿海沉积物样品中含有丰富多样的海洋放线菌,大量菌株具有抗菌与; 抗肿瘤活性,是天然产物开发的重要资源。

Objective To find new lead compounds, the diversity, antimicrobial and; antitumor activities of marine actinobacteria from marine sediments of; Fujian coast were investigated. Methods Actinobacteria strains were; selective isolated from 15 marine sediments of Fujian coast. The 16S; rRNA gene sequences analysis of representative strains were carried out; to evaluate their diversity. All of the isolates were assessed using 10; screening models for their bioactivities. Results A total of 216 marine; actinobacteria were isolated by using four media. The isolation effect; of MOPS-proline medium was the best choice. Phylogenetic analysis based; on 16S rRNA gene sequences showed that 77 representative strains; belonged to 14 genera, and there were 10 strains identified as potential; novel species. One hundred and sixty-four (77%) strains showed; antimicrobial activity against at least one kind of microorganisms.; Thirty-nine (18.1%) strains showed cytotoxic activity with inhibition; rate above 50% against Hela or HepG2 cells in vitro. Conclusion This; study indicated that actinobacteria from marine sediments of Fujian; coast are abundant, many of them had antimicrobial and cytotoxic; activities, which can be further explored.