SERS技术应用于食品中罗丹明B的快速检测

Abstract

目的:罗丹明B(Rhodamine; B,RB)是一种人工合成的三苯甲烷类碱性染料,因其具有潜在的毒性、致癌性被禁止在食品中添加。目前,在中国还没有关于罗丹明B检测的国家标准,也没有; 快速检测标准,建立合适的现场快速检测方法迫在眉睫。方法:借助表面增强拉曼光谱(SERS)技术,以金纳米溶胶为拉曼信号增强模块,利用自制样品前处理; 仪与便携式拉曼光谱检测仪,实现对辣椒制品、腊肉、果汁、葡萄酒等食品中RB的快速检测。结果:快速前处理技术与常规方法相比,SERS谱图结果差异不明; 显,且不受样品其他成分干扰,但分析时间由常规的40~50 min缩短到约10; min,大大提高了检测效率;同时,以样品提取液为基质的RB溶液最低检测浓度可以达到0.5 mg /kg;以RB在1355; cm~(-1)处的特征峰为内标信号进行归一化计算,在100、200、300 mug; /L三个添加水平下,得到其平均回收率在81.1%~87.0%之间,相对标准偏差RSD在2.1%~3.0%内。结论:通过对多种实际样品检测并将结果; 与成熟的实验室液相色谱法相比较,发现本检测方法样品前处理简单,耗时少,检测结果准确可靠,且仪器设备便携、易操作,可用于食品中RB的现场快速筛查或; 实验室预检。

Objective: Rhodamine B is one kind of synthetic alkaline; triphenylmethane dye,which have been used as a food additive.But now it; is proved to be a toxic and cancer-causing substance,and is forbidden to; artificially add into food in China and EU. At present,the government; has not enacted relevant testing standards,and no on-site test methods; was put forward. Therefore it is imminent to establish an on-site rapid; detection method.Method: Based on surface enhanced Raman spectroscopy; (SERS)technique,this experiment achieved the rapid detection of; rhodamine B in chilli products,such as bacon,fruit juice, wine and other; foods,used gold nanoparticle sol as Raman signal enhancement module,a; home-made laser Raman spectra pretreatment apparatus and a portable; Raman spectrum detector.Result: Compared with the conventional; methods,the difference of SERS spectra was not obvious,and the results; also were not affected by the sample composition,though the experimental; time was shorten from 40~50 min to about 10 min,and the minimum; detection concentration for RB was 0.5 mg /kg.By using the intensity of; 1355cm~(-1) peak as a normalization standard,the recoveries for chili; spikes(100,200,300 mug /L)in chilli powder ranged from 81.1% ~87.0% and; the relative standard deviations(RSD)from 2.1% to 3.0%.Conclusion: The; actual samples purchased from the supermarket were tested by the quick; method mentioned in this study.The results told us that this method was; reliable compared with proven technique of LC,and the device used in; this experiment is portable and easy to be operated, and the results; also showed us that this method can be applied for site inspection and; preliminary inspection in laboratory.