Multifunction of Ribosome-inactivating Protein from Bougainvillea glabra Choisy Showed in Transgenic Tomato
- 生命科学－已发表论文 
[中文文摘]根据已报道的三角梅(Bougainvillea spectabilis Willd)的核糖体失活蛋白(RIP)基因序列,以厦门本地艳紫三角梅(B. glabra Choisy cv. Sanderiana)为材料,通过RT-PCR技术扩增到其RIP成熟蛋白的基因序列BouM并成功转化番茄.经检测,BouM转基因番茄对蚜虫、蚂蚁及番茄灰霉菌都有很好的抗性作用,显示了其在植物抗病抗虫中的应用前景.但是同时转基因番茄对小白鼠的生长有一定的抑制作用,因此将其作为植物抗病虫基因利用时,其生物安全性也是一个值得重视的问题.[英文文摘]Bougain villea mature ribo some inactivating protein(RIP) are supposedly the least toxic. Its enzymatic activities, toxic to cancer cells or HIV and ant ivirus activity in plant have already been reported. In this study, gene sequence of RIP from Bougainvillea glabr a Choisy cv. Sanderiana, named as BouM was cloned and transferred to tomato . Results showed that transgenic plants of BouM are resistant to aphid, ant and Botrytis cinerea. These results imply a huge application potential of Boug ainvillea RIP in improving plant resistance. On t he other hand, it seems that there is still a safe problem in the application of Boug ainvillea RIP because transgenic plants of BouM are found to be toxic to laboratory mouse to some degree in this research.