DETERMINATION OF SPARTINA ANGLICA STOCK COMPONENTS BY NREL METHOD
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采用美国国家可再生能源实验室(NREL)方法定量大米草原料中纤维素、半纤维素及木质素。72%浓硫酸水解1 h、4%稀硫酸水解1 h可将大米草的纤维素、半纤维素降解为可用HPLC定量的单糖,适宜的样品添加量为0.3 g。同时,NREL法测定大米草纤维素、半纤维素及木质素的含量分别为32.92%、27.65%和24.2%。这三个组分的含量是评价大米草预处理、酶解及发酵工艺条件的重要依据。Cellulose, hemicellulose and lignin of Spartina anglica were quantified by National Renewable Energy Lab(NREL) method. It was showed that the cellulose and semicellulose of S. anglica could be degraded to mono-sugars which could be quantified by HPLC after it was hydrolyzed with 72% concentrated sulphuric acid for 1 h and 4% dilute sulphuric acid for 1h with the appropriate additive amount of 0.3 g sample. Meanwhile, the contents of cellulose, hemicellulose and lignin of S. anglica determined by NREL method were 32.92%, 27.65%and 24.2% respectively. The contents of these three fractions are important basis for the evaluation of the pre-processing, enzymatic hydrolysis and fermentation conditions of S. anglica.