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dc.contributor.author洪志聪
dc.contributor.author骆献阳
dc.contributor.author蔡成福
dc.contributor.author徐剑
dc.contributor.author庄国顺
dc.date.accessioned2018-11-26T07:28:06Z
dc.date.available2018-11-26T07:28:06Z
dc.date.issued2017-09-28
dc.identifier.citation中南大学学报. 医学版,2017,(9):1042-1047
dc.identifier.issn1672-7347
dc.identifier.other10.11817/j.issn.1672-7347.2017.09.008
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/163004
dc.description.abstract目的:研究大气细颗粒物(fine particulate; matter,PM_(2.5))对人支气管上皮细胞活性的影响及其炎性作用。方法:用PM_(2.5)采样器采集上海地区大气PM_(2.5)样本,扫; 描电镜观察PM_(2.5)形态特征。将人支气管上皮细胞BEAS-2B暴露于不同浓度(0,50,100,200,400,800; mug/mL)的PM_(2.5) 12,24,48 h,细胞活力检测试剂盒(cell counting kit-8,; CCK-8)法检测PM_(2.5)暴露对细胞活性的影响。实时定量PCR(quantitative real-time; PCR,qRT-PCR)检测细胞粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating; factor,GM-CSF)和TNF-alpha mRNA的表达,Western印迹检测; GM-CSF和TNF-alpha蛋白的表达。结果:扫描电镜观察发现,PM_(2.5)形态多样,大小不一,直径大多等于或小于2.5; mum。与同时间点未暴露组比较,各暴露组(50~800; mug/mL)细胞活性呈不同程度的下降,差异具有统计学意义(P<0.05)。与未暴露组比较,暴露于100,400或800 mug/mL; PM_(2.5) 24 h后,GM-CSF和TNF-alpha; mRNA和蛋白表达水平明显升高(P<0.05),且PM_(2.5)暴露浓度越高,GM-CSF和TNF-alpha的mRNA和蛋白升高水平越显著。; 结论:大气PM_(2.5)可引起人支气管上皮细胞的炎症反应,降低细胞活性,这可能与PM_(2.5)促发和加重支气管肺部炎性疾病有关。
dc.description.abstractObjective: To investigate the effects of airborne fine particle on cell; viability and inflammation in human bronchial epithelial cells. Methods:; Atmospheric PM_(2.5) samples were collected by PM_(2.5) sampler.; PM_(2.5) morphology was observed by scanning electron microscope (SEM).; Human bronchial epithelial cells (BEAS- 2B) were treated with PM_(2.5); at different concentrations (0, 50, 100, 200, 400, 800 mug/mL) for 12,; 24 or 48 hours, and the cell activity were evaluated by cell counting; kit-8 (CCK-8). The mRNA expression levels of (granulocyte-macrophage; colony stimulating factor,GM-CSF) and TNF-alpha were detected by; quantitative real-time PCR (qRT-PCR). Western blot was used to detect; the protein expressions of GM-CSF and TNF-alpha. Results: According to; SEM, the shape of PM_(2.5) varied, and the diameter was different and; mostly equal to or less than 2.5 mum. CCK-8 assay showed that different; concentrations of PM_(2.5) exposure for 12 hours, 24 hours and 48 hours; resulted in loss of cell viability of BEAS-2B cells (P<0.05). Different; concentrations of PM_(2.5) increased the mRNA and protein expression of; GM-CSF and TNF-alpha, and the higher concentration of PM_(2.5) induced; higher expression, which have statistical significant difference between; the groups (P<0.05). Conclusion: Atmospheric PM_(2.5) can cause; inflammatory response in human bronchial epithelial cells. They can; reduce cell viability, which may be related to the PM_(2.5) trigger and; aggravation of bronchopulmonary inflammatory diseases.
dc.language.isozh_CN
dc.subject大气细颗粒物
dc.subject人支气管上皮细胞
dc.subject炎性
dc.subject细胞活性
dc.subjectairborne fine particle
dc.subjecthuman bronchial epithelial cells
dc.subjectinflammation
dc.subjectcell viability
dc.title大气细颗粒物对人支气管上皮细胞的活性抑制和炎性作用
dc.title.alternativeAirborne fine particle decreases the cell viability and induces inflammation in human bronchial epithelial cells
dc.typeArticle


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