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dc.contributor.author谢友坪
dc.contributor.author赵旭蕊
dc.contributor.author阳需求
dc.contributor.author卢英华
dc.contributor.author郑向南
dc.contributor.author陈剑锋
dc.date.accessioned2018-11-26T07:26:58Z
dc.date.available2018-11-26T07:26:58Z
dc.date.issued2017-07-25
dc.identifier.citation食品科学,2017,(14):64-70
dc.identifier.issn1002-6630
dc.identifier.other10.7506/spkx1002-6630-201714010
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/162912
dc.description.abstract考察脉冲式添加氮源培养对耐温微藻Desmodesmus sp.; F51细胞生长和细胞组成的影响。结果表明,在脉冲式添加氮源培养过程中,藻株F51的蛋白质含量由(56016)mg/g降低至(45617)mg/g; ,而碳水化合物和油脂含量则分别由(20913)mg/g和(983); mg/g提高至(31012)mg/g和(1204)mg/g。另外,对色素和油脂的具体组成变化进行分析,发现脉冲式添加氮源培养可增强beta-胡萝; 卜素的生物合成,以及促进alpha-胡萝卜素生物转化为叶黄素,同时还可发现叶黄素积累与多不饱和脂肪酸含量变化呈正相关性。而在碳水化合物的具体组成; 分析中,发现碳水化合物为藻株F51主要的储能物质,当脉冲式添加氮源培养时间为3; d时,其葡萄糖占总碳水化合物的质量分数可明显提高至(75.71.4)%。采用脉冲式添加氮源培养策略可明显同时促进藻株F51叶黄素和碳水化合物的生; 产。
dc.description.abstractThe aim of the present study was to investigate the effect of nitrate; pulse feeding on the cell growth and cell composition of Desmodesmus sp.; F51. Under nitrate pulse-feeding cultivation, the protein content of; cells of Desmodesmus sp. F51 decreased from(560 16) mg/g to(456 17); mg/g, while the contents of carbohydrate and lipid increased from(209 ; 13) mg/g to(310 12) mg/g and(98 3) mg/g to(120 4) mg/g, respectively.; By investigating the changes in carotenoid, lipid and carbohydrate; compositions, it appeared that nitrate pulse feeding could promote; beta-carotene biosynthesis and enhance the bioconversion of; alpha-carotene to lutein. Lutein accumulation was positively associated; with polyunsaturated fatty acid formation. Desmodesmus sp. F51 tended to; accumulate carbohydrate as an energy storage product after 3 days of; cultivation under nitrate pulse-feeding conditions, and the major; accumulated monosaccharide was glucose(75.7 1.4)%. Therefore, the; nitrate-pulse feeding strategy is a highly promising approach to; simultaneously produce lutein and carbohydrate in Desmodesmus sp. F51.
dc.description.sponsorship国家自然科学基金应急管理项目; 福建省青年科技人才创新基金资助项目; 福建省海洋高新产业发展专项; 福州市市校(院所)科技合作项目
dc.language.isozh_CN
dc.subject微藻
dc.subject流加培养
dc.subject细胞组成
dc.subject叶黄素
dc.subject碳水化合物
dc.subjectmicroalgae
dc.subjectfed-batch culture
dc.subjectcell composition
dc.subjectlutein
dc.subjectcarbohydrate
dc.title脉冲式添加氮源对耐温微藻Desmodesmus sp.F51细胞生长和细胞组成的影响
dc.title.alternativeEffect of Nitrate Pulse-Feeding Cultivation on Cell Growth and Cell Composition of Thermo-Tolerant Desmodesmus sp. F51
dc.typeArticle


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