Determination of Benzo[a]Pyrene in Vegetables and Grain Products by Derivative Synchronous Fluorescence Spectroscopy
- 化学化工－已发表论文 
结合导数恒能量同步荧光扫描技术,采用标准加入法定量检测,导数基线校正法处理数据,建立了一种蔬菜及谷物制品中苯并（α）芘的荧光快速检测方法。一般样品仅需简单超声萃取,深色蔬菜及在370 nm左右有干扰峰的谷物制品也只需增加微波皂化步骤,即可进行光谱扫描。对实际样品高（10μg/kg）、中（5.0μg/kg）、低（1.0μg/kg）浓度的加标回收率在84.2%~103.2%之间,方法重现性好,各类样品的检出限为0.17~0.55μg/kg,标准加入法工作曲线线性范围为0.034~50μg/L。该方法样品前处理简单,无需复杂的纯化分离步骤,适用于蔬菜及谷物制品中苯并（α）芘的快速分析测定。Coupling derivative constant-energy synchronous fluorescence scanning technique with standard addition method and derivative baseline correction for data procession, a rapid and sensitive quantitative detection method for benzo[ α ]pyrene in vegetables and grain products was established. Simple uhrasnnic extraction was the only pretreatment procedure for general samples, and the dark vegetable and grain samples which had interference peak at 370 nm should be pre-processed with saponification before the analysis. It was applied to determine benzo[ α]pyrene in vegetables and grain products with good reproducibility and the recoveries of different spiking concentrations （10, 5.0, 1.0 μ g/kg） ranged from 84.2%-103.2%. The limits of detection were between 0.17-0.55 μ g/kgwith linear range from 0.034-50 IX g/L. This method was simple, rapid, inexpensive and accurate without complex purification separation steps. It was suitable to the rapid determination of benzo[ α ]pyrene in vegetables and grain products.