Tolerance of Gill Cells in Primary Cultured Oyster to Zinc Ion
本文以福建牡蛎为实验材料,探索体外原代细胞培养方法,测试体外培养细胞对锌离子的耐受特性。结果表明,以pH7.25、渗透压摩尔浓度为1000mosm、10%透析胎牛血清的L-15优化培养基,能顺利的在体外培养牡蛎鳃细胞且细胞活力在80%以上;原代牡蛎腮细胞对锌离子暴露的耐受特征与牡蛎对锌离子的耐受特征高度相似,24、48、72、96小时的半致死浓度分别为239.47、72.60、12.13和8.19mg·L~（-1）,表明体外培养的牡蛎鳃原代细胞维持了原有的锌耐受特性。In the paper, Fujian oysters were used as experimental materials to explore the primary cell culture methods in vitro and to test the tolerance of cells to zinc ions in vitro. The results showed that the activity of oyster gill cells and the cell viability were more than 80% in the L-15 optimized medium with pH7.25, osmolality of 1000 mosm and 10% dialysis fetal bovine serum. The tolerance to zinc exposure was similar to that of oyster, and the half lethal concentrations at 24, 48, 72 and 96 hours were 239.47, 72.60, 12.13 and 8.19 mg · L-1, respectively. Cultured oyster gill primary cells maintained the original zinc tolerance characteristics.