Cytological Observation on Cross Fertilization of Halilotis discus hannai and Halilotisgigantea with Fluorescent Microscope
- 海洋环境－已发表论文 
本研究采用荧光显微技术对皱纹盘鲍（Halilotis discus hannai）♀×西氏鲍（H.gigantea）♂的受精过程及受精卵的早期发育进行了观察,并采用FITC-anti-α-tubulin对精子入卵位置进行标记.结果显示西氏鲍的精子可正常激活皱纹盘鲍的卵子,精子进入卵子2-5 min后受精卵开始第一次卵裂,释放出第一极体.随着受精卵的发育卵子完成第二次减数分裂,排出第二极体,同时形成早期精卵原核.精卵原核膨胀并彼此融合,受精卵开始进行有丝分裂完成第一次卵裂.通过FITC标记的anti-α-tubulin蛋白免疫荧光观察,发现精子在入卵时会在卵膜表面,形成一个类似于受精孔的结构.The current study was conducted on the fertilization process and the early development of fertilized eggs by the fluorescence microscopy technique. The position of sperm into the eggs was labeled by FITC - anti - α - tubulin. The results showed that sperm of H. gigantea penetrated H. discus hannai eggs. The first cleavage and the first polar body were released in after 2 -5min of sperm - egg fusion. With the completion of the second meiotic division of the fertilized egg, the second polar body was released, forming the early male and female pronucleus. Then two pronucleus began to swell and started mutual fusion, and the fertilized eggs began to undergo mitosis to complete the first cleavage. The immunofluorescent observation with the FITC labeled anti - α - tubulin probe detected a pore structure similar to micropylar on the surface of egg membrane.