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dc.contributor.author陈晔
dc.contributor.author陈荣
dc.contributor.author莫正平
dc.date.accessioned2018-11-26T07:23:56Z
dc.date.available2018-11-26T07:23:56Z
dc.date.issued2016-06-15
dc.identifier.citation生态毒理学报,2016,11(03):127-133
dc.identifier.issn1673-5897
dc.identifier.otherSTDL201603013
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/162417
dc.description.abstract为了解重金属污染对海洋鱼类热休克蛋白(HSPs)基因表达的影响,将褐菖鲉(Sebastiscus marmoratus)分别暴露于1.6、8、40、200、500μg·L~(-1)Cd、Pb溶液中,用环介导等温扩增技术(loop-mediated isothermal amplification,LAM P)定量检测褐菖鲉肝脏HSP60、HSP70、HSP90、HSC70 mRNA表达量。结果表明:Pb仅在40μg·L-1时显著抑制HSP60、HSP90、HSC70 mRNA表达量,8μg·L~(-1)时即可显著抑制HSP70 m RNA表达量,并在40μg·L~(-1)时达到最小值;Cd对HSP60、HSP90、HSC70的诱导不明显,但能显著诱导HSP70,并在500μg·L~(-1)时达到最大值。相比之下,褐菖鲉肝HSP70基因对重金属Cd、Pb污染较为敏感,有潜力成为监测海洋重金属污染的预警分子。
dc.description.abstractIn order to understand the effects of heavy metal pollution on the gene expression of heat shock proteins(HSPs) in marine fish,Sebastiscus marmoratus were exposed to different concentrations(i. e.,1. 6,8,40,200,500 μg·L~(-1)) of Cd or Pb for 5 days. A quantitative loop-mediated isothermal amplification(LAMP) method was used to analyze the hepatic mRNA levels of HSP60,HSP70,HSP90,and HSC70. Under the Pb exposure,the m RNA levels of hepatic HSP60,HSP90,HSC70 were not significantly reduced except in the 40 μg·L~(-1) treatment,whereas the m RNA levels of HSP70 were significantly decreased by 8 μg·L-1Pb,and reached the minimum when Pb was 40 μg·L~(-1). Under the Cd exposure,the m RNA levels of hepatic HSP60,HSP90,HSC70 were not significantly affected;in contrast,m RNA levels of HSP70 were significantly increased and peaked at 500 μg·L-1Cd. In conclusion,the hepatic HSP70 was relatively more sensitive to heavy metal exposure than the other HSPs,and thushad the potential to be the early warning for heavy metal pollution in marine environment.
dc.description.sponsorship海洋公益性项目"海洋污染生物效应快速监测与评价技术应用示范"子任务-海洋污染效应生物标志物技术集成与研发Ⅱ(201005016)
dc.language.isozh_CN
dc.subjectCd
dc.subjectPb
dc.subject褐菖鲉
dc.subject肝脏
dc.subject热休克蛋白
dc.subjectHSPs
dc.subject环介导等温扩增
dc.subjectCd
dc.subjectPb
dc.subjectSebastiscus marmoratus
dc.subjectliver
dc.subjectheat shock proteins
dc.subjectHSPs
dc.subjectloop-mediated isothermal amplification
dc.title重金属Cd、Pb对褐菖鲉肝脏组织中HSPs基因表达的影响
dc.title.alternativeThe Effects of Cd and Pb on the Levels of Hepatic HSPs mRNA in Sebastiscus marmoratus
dc.typeArticle


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