The Effects of Cd and Pb on the Levels of Hepatic HSPs mRNA in Sebastiscus marmoratus
- 海洋环境－已发表论文 
为了解重金属污染对海洋鱼类热休克蛋白(HSPs)基因表达的影响,将褐菖鲉(Sebastiscus marmoratus)分别暴露于1.6、8、40、200、500μg·L~(-1)Cd、Pb溶液中,用环介导等温扩增技术(loop-mediated isothermal amplification,LAM P)定量检测褐菖鲉肝脏HSP60、HSP70、HSP90、HSC70 mRNA表达量。结果表明:Pb仅在40μg·L-1时显著抑制HSP60、HSP90、HSC70 mRNA表达量,8μg·L~(-1)时即可显著抑制HSP70 m RNA表达量,并在40μg·L~(-1)时达到最小值;Cd对HSP60、HSP90、HSC70的诱导不明显,但能显著诱导HSP70,并在500μg·L~(-1)时达到最大值。相比之下,褐菖鲉肝HSP70基因对重金属Cd、Pb污染较为敏感,有潜力成为监测海洋重金属污染的预警分子。In order to understand the effects of heavy metal pollution on the gene expression of heat shock proteins(HSPs) in marine fish,Sebastiscus marmoratus were exposed to different concentrations(i. e.,1. 6,8,40,200,500 μg·L~(-1)) of Cd or Pb for 5 days. A quantitative loop-mediated isothermal amplification(LAMP) method was used to analyze the hepatic mRNA levels of HSP60,HSP70,HSP90,and HSC70. Under the Pb exposure,the m RNA levels of hepatic HSP60,HSP90,HSC70 were not significantly reduced except in the 40 μg·L~(-1) treatment,whereas the m RNA levels of HSP70 were significantly decreased by 8 μg·L-1Pb,and reached the minimum when Pb was 40 μg·L~(-1). Under the Cd exposure,the m RNA levels of hepatic HSP60,HSP90,HSC70 were not significantly affected;in contrast,m RNA levels of HSP70 were significantly increased and peaked at 500 μg·L-1Cd. In conclusion,the hepatic HSP70 was relatively more sensitive to heavy metal exposure than the other HSPs,and thushad the potential to be the early warning for heavy metal pollution in marine environment.