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dc.contributor.authorZhang, Wei
dc.contributor.authorLi, Xue-Jun
dc.contributor.authorZeng, Xin
dc.contributor.authorShen, Dong-Yan
dc.contributor.author沈东炎
dc.contributor.authorLiu, Chang-Qin
dc.contributor.authorZhang, Hui-Jie
dc.contributor.authorXu, Cang-Bao
dc.contributor.authorLi, Xiao-Ying
dc.date.accessioned2013-04-22T09:24:07Z
dc.date.available2013-04-22T09:24:07Z
dc.date.issued2012-03-07
dc.identifier.citationTOXICOLOGY LETTERS,2012,209(2):107-112zh_CN
dc.identifier.issn0378-4274
dc.identifier.urihttp://dx.doi.org/10.1016/j.toxlet.2011.12.005
dc.identifier.uriWOS:000301277200001
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/16172
dc.description.abstractThe endothelin B2 (ETB2) receptors are induced in vascular smooth muscle cells (VSMCs) in cardiovascular diseases. We tested if in vitro short-term exposure to the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could up-regulate ETB2 receptors in rat mesenteric arteries, and if this effect is through activation of intracellular nuclear factor-kappa B (NF-kappa B) pathway. The mesenteric arteries were dissected from male Sprague-Dawley rats and the endothelium was removed. The arteries were co-incubated with TNF-alpha in serum-free Dulbecco's modified Eagle's medium. Real-time reverse transcription-PCR, Western blot and immunohistochemical staining were employed to assess the mRNA/protein expression of ETB2 receptors and activation of NF-kappa B pathway. The results showed that, during organ culture. TNF-alpha concentration-dependently enhanced ET52 receptors expression at both mRNA and protein levels, paralleled with activation of NF-kappa B pathway in VSMC. The up-regulated ETB2 receptor expression and NF-kappa B activation could be effectively suppressed by general transcriptional inhibitor actinomycin D, or either of the selective I kappa B kinase inhibitors wedelolactone and IMD-0354. Conclusively, the activation of intracellular NF-kappa B pathway is responsible for the up-regulation of ETB2 receptors induced by short-term exposure to TNF-alpha. This could partly explain the toxic effects of TNF-alpha on VSMCs that account for cardiovascular diseases. (C) 2011 Elsevier Ireland Ltd. All rights reserved.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [81100194, 81000320]; Excellent Youth Foundation of Fujian Province, PR China [2009D016]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER IRELAND LTDzh_CN
dc.subjectTumor necrosis factor-alphazh_CN
dc.subjectEndothelin B2 receptorzh_CN
dc.subjectNuclear factor-kappa Bzh_CN
dc.subjectVascular smooth muscle cellzh_CN
dc.titleActivation of nuclear factor-kappa B pathway is responsible for tumor necrosis factor-a-induced up-regulation of endothelin B2 receptor expression in vascular smooth muscle cells in vitrozh_CN
dc.typeArticlezh_CN


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