Preparation and Characteristic Research of Immobilized Acetylcholinesterase
- 化学化工－已发表论文 
A new method for preparation of a reusable immobilized acetylcholinesterase (AchE) was developed in order to lay a foundation for screening AchE inhibitors from natural products. AchE was immobilized on aminated silica gel microspheres with glutaraldehyde as cross-linking reagent. The optimum conditions and properties of immobilized enzyme were investigated. The optimal immobilization process was as follows 0.05 g of aminated silica gel microspheres was activated by glutaraldehyde solution for 6 h, and mixed with AChE solution with the activity of 5 U. Then, the mixture was shook for 16 h at 28 degrees C. The immobilized enzyme produced by this method had the highest enzyme activity, and it could remain better activity witha wide range of pH (pH 6 similar to 10) at room temperature (20 similar to 40 degrees C). The immobilized AChE showed higher storage stability and could be used repeatedly.