Apoptosis of Thymosin on Hepatocellular Carcinoma Cell HepG_2 and Discussion of Its Mechanism
- 医学院－已发表论文 
以 MTT法检测胸腺肽对 Hep G2 的细胞毒作用 ,以细胞原位凋亡染色法 (Tunel法 )检测胸腺肽诱导 Hep G2 细胞凋亡 ,并以 western- blot法及免疫组化法检测药物处理后 bcl- 2、Fas抗原的表达变化 .结果显示在 0～ 150 μg/ m L浓度范围内 ,胸腺肽对 Hep G2 无明显的细胞毒作用 ,当药物浓度超过 150 μg/ m L以上 ,细胞毒作用呈上升趋势 ,其 IC50 为 330 μg/ m L,而低于 150 μg/ m L 的胸腺肽对Hep G2 细胞有诱导凋亡的作用 ,并能下调 bcl- 2的表达量 ,同时增加 Fas抗原的表达 .结论 :大剂量国产胸腺肽对 Hep G2 细胞生长有抑制作用 ,低剂量胸腺肽在一定程度上可诱导 Hep G2 肝癌细胞的凋亡 ,其机制可能是通过 Fas介导的途径Cytotoxicity of thymosin on HepG 2 cell lines was measured by MTT assay, and apoptosis of HepG 2 induced by thymosin was observed by Tunel assay; immunochemistry SP asssay and Western blot were used to measure the expression changes of Fas and Bcl 2, respectively, after treating by thymosin. Results showed that thymosin had low cytotoxicity on HepG 2 within the range of 0~150 μg /mL, however the cytotoxicity was sharply increased when the thymosin concentration was higher than 150 μg/mL. The IC 50 is 330 μg/mL. With thymosin concentration at 12.5~100 μg/mL, apoptosis of HepG 2 was observed, meanwhile Fas antigan increased and Bcl 2 protein decreased. It can be concluded that: at the range of 12.5~150 μg/mL, thmyosin induces apoptosis of hepatocellular carcinoma cell HepG 2 to a certain extent. It is possible that the Fas mediated pathway is taken during apoptosis.