Inhibition of Sinogen on HBV Replication of HepG2. 2. 15 Cell line in Vitro
- 医学院－已发表论文 
以转染有ＨＢＶ的ＨｅＰＧ２．２．１５细胞株为实验模型，用ＭＴＴ法测定赛若金对２．２．１５细胞的生长抑制作用，流式细胞仪测定药物对２．２．１５细胞周期时相的改变，通过ＥＬＩＳＡ方法观察赛若金对２．２．１５细胞分泌ＨＢｅＡｇ及ＨＢｓＡｇ的影响，ＰＣＲ－Ｈｙｂ杂交法检测赛若金对２．２．１５细胞ＨＢＶＤＮＡ复制的抑制作用．结果显示，赛若金对转染乙肝病毒的２．２．１５细胞有生长抑制作用，抑制率随药物浓度呈剂量依赖关系，并且在药物量达 ３ ２００ ＩＵ／ｍＬ后趋于衡定，其ＩＣ５０为 ４ ０７８．５ ＩＵ／ｍＬ．流式细胞仪分析结果显示，赛若金主要抑制２．２．１５细胞生长周期的Ｇ１期和Ｇ２－Ｍ期。抗ＨＢＶ复制实验显示当赛若金 １２００ ＩＵ／ｍＬ时，对 ２．２．１５细胞株 ＨＢｓＡｇ分泌高度抑制，抑制率达 ９０％以上，同时对ＨＢｅＡｇ和 ＨＢＶ ＤＮＡ也有部分抑制作用，它们的抑制率分别为 ６７％和 ７０％左右，HepG2. 2. 15 cell line, transfected with HBV DN,was used as an experiment mod- el. MTT assay was employed to detect the growth inhibition of 2. 2.15 cells treated by Sinogen. The change of the cell cycle was measured by flow cytometry technique (FCM). The effects of Sinogen on the secret HBeAg, HBsAg, as well as HBVDNA, were assayed by ELISA method and PCR-Hybridization, respectively. The results showed that the growth inhibition of 2. 2. 15 cells was observed after treatment of Sinogen. The inhibition rate was dose-dependent and reached maximum when the treated concentration reached 3 200 IU/mL. Its IC50 was 4 078. 5 IU/mL. FCM assay showed that 2.2.15 cell's cycle was arrested on G1 and G2-M phage. The HBsAg secreting was inhibited by 90%, in the meantime, HBeAg and HBV DNA were partially inhibited by Sinogen. Their inhibition rates were 50% and 60%, respectively.