The Prevalence of HEV among Pigs and Cattle
- 厦门大学－已发表论文 
目的 探讨戊型肝炎病毒(HEV)在猪和牛群中的感染情况。方法 用抗-HEV抗体试剂盒检测猪和牛血清中的抗体;对部分血清用逆转录聚合酶链的方法(RT-PCR)检测HEV RNA,并对PCR阳性产物进行克隆测序,然后将序列进行分析。结果 281份猪血清中有235份为抗-HEV抗体阳性,阳性率为83.6％;其中56份血清中有3份为HEV RNA阳性,序列分析显示从猪中克隆的3株序列(SB 2、SH1和SJ 14)之间在ORF 2(6007-6354bp)区域的核苷酸序列的同源性为83％～86％,该3株序列在ORF 2区与1,2,3,4型的同源性分别为74％～79％、73％～77％,73％～76％和83％～93％;190份牛血清中有12份为抗-HEV抗体阳性,阳性率为6.3％。结论 HEV在猪和牛群中均有流行,但在猪群中的流行率明显高于牛群。猪感染的HEV的基因序列与人群中散发性戊型肝炎病毒的4型同源性最高。Objective To investigate prevalence of HEV in pigs and cattle. Methods The and - HEV antibody was detected in sera collected from pigs and cattle with anti - HEV EIA.HEV RNA was detected using RT- PCR with ORF 2 primers.The positive PCR products were cloned and sequenced.The sequences were compared with the reported HEV isolates. Results 235 of 281 pigs' sera were positive for anti - HEV antibody.3 of 56 pigs sera were positive for HEV RNA.The sequence analysis showed that the identity at nucleotide level was 83% - 86% among them.They had 74% - 79%, 73% - 77%, 73% - 76% and 83% - 93% identity to genotypes 1, 2, 3 and 4 respectively in this region. 12 of 190 cattle' s sera were positive for anti - HEV antibody. Conclusion The prevalence of HEV is much higher in pigs than in cattle. HEV sequences isolated from pigs belong to HEV 4 isolated from patients with acute hepatitis.