Now showing items 107-126 of 719

    • Rab34 regulates adhesion, migration, and invasion of breast cancer cells 

      Lixiang Sun; 孙礼祥; Xiaohui Xu; 许晓慧; Yongjun Chen; Yuxia Zhou; Ran Tan; Hantian Qiu; Liting Jin; Wenyi Zhang; Rong Fan; Wanjin Hong; 洪万进; Tuanlao Wang; 王团老 (Macmillan Publishers Limited,part of Springer Nature., 2018-04)
      【Abstract】The small GTPase Rab34 regulates spatial distribution of the lysosomes, secretion and macropinocytosis. In this study, we found that Rab34 is over expressed in aggressive breast cancer cells, implying a potential ...
    • Rab39A多克隆抗体的制备与鉴定 

      马琳琳; 张明; 杨婷; 王卫杰; 王团老 (2013-01-28)
      制备抗人Rab39A多克隆抗体并进行纯化检测,以用于Rab39A功能的研究.选取免疫原性强且特异性高的羧基端42个氨基酸(176~217aa)作为目的片段.PCR法扩增并构建重组表达质粒pGEX-4T-1-Rab39C42.异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达并纯化GST-Rab39C42融合蛋白,免疫新西兰兔,制备多克隆抗体.然后以亲和层析法纯化抗体,利用Western blot和免疫荧光检测抗体的特异性.结果表明,构建的 ...
    • RXR agonists inhibit high-glucose-induced oxidative stress by repressing PKC activity in human endothelial cells 

      Chai, Dajun; Wang, Binyao; Shen, Linghong; Pu, Jun; Zhang, Xiao-Kun; He, Ben; 张晓坤 (FREE RADICAL BIO MED, 2008-04-01)
      Activation of retinoid X receptor (RYR) is known to exert antiatherogenic effects. However, the underlying mechanism remains unclear. In this study.. we examined the effects of the RXR agonists 9-cis-retinoic acid and ...
    • SBA-15与SBA-16作为药物载体的性能研究 

      郑宗富; 林赵栋; 郑梅淋; 洪宇; 翁少煌; 谭鸿 (2015-6-15)
      目的:通过建立Sd大鼠2型糖尿病模型的方法,研究不同的介孔材料SAb-15与SAb-16分别载药格列齐特(glz),释放速率的作用,以筛选出最佳的载药系统,为临床制剂提供参考。方法:考察SAb-15/glz、SAb-16/glz的载药、释药性能,建立大鼠糖尿病模型,分别检测灌胃给药前(0H)和给药后各时间点(3 H,6 H,9 H,12 H,24 H,27 H,30 H,48 H)各组大鼠血糖浓度的变化。结果:1载药系统的热重分析,Sb ...
    • Small GTPase Rab40c Associates with Lipid Droplets and Modulates the Biogenesis of Lipid Droplets 

      Tan, Ran; Wang, Weijie; Wang, Shicong; Wang, Zhen; Sun, Lixiang; He, Wei; Fan, Rong; Zhou, Yunhe; Xu, Xiaohui; Hong, Wanjin; Wang, Tuanlao; 洪万进; 王团老 (PUBLIC LIBRARY SCIENCE, 2013-Apr 3)
      The subcellular location and cell biological function of small GTPase Rab40c in mammalian cells have not been investigated in detail. In this study, we demonstrated that the exogenously expressed GFP-Rab40c associates with ...
    • Sulindac-Derived RXR alpha Modulators Inhibit Cancer Cell Growth by Binding to a Novel Site 

      Chen, Liqun; Wang, Zhi-Gang; Aleshin, Alexander E.; Chen, Fan; Chen, Jiebo; Jiang, Fuquan; Alitongbieke, Gulimiran; Zeng, Zhiping; Ma, Yue; Huang, Mingfeng; Zhou, Hu; Cadwell, Gregory; Zheng, Jian-Feng; Huang, Pei-Qiang; Liddington, Robert C.; Zhang, Xiao-kun; Su, Ying; 曾志平; 周虎; 郑剑峰; 黄培强; 张晓坤 (CELL PRESS, 2014-05-22)
      Retinoid X receptor-alpha (RXR alpha), an intriguing and unique drug target, can serve as an intracellular target mediating the anticancer effects of certain nonsteroidal anti-inflammatory drugs (NSAIDs), including sulindac. ...
    • TAZ Expression as a Prognostic Indicator in Colorectal Cancer 

      Yuen, Hiu-Fung; McCrudden, Cian M.; Huang, Yu-Han; Tham, Jill M.; Zhang, Xiaoqian; Zeng, Qi; Zhang, Shu-Dong; Hong, WanJin; 洪万进 (PUBLIC LIBRARY SCIENCE, 2013-Jan 2)
      The Hippo pathway restricts the activity of transcriptional coactivators TAZ (WWTR1) and YAP. TAZ and YAP are reported to be overexpressed in various cancers, however, their prognostic significance in colorectal cancers ...
    • Tethering the assembly of SNARE complexes 

      Hong, WanJin; Lev, Sima; 洪万进 (ELSEVIER SCIENCE LONDON, 2014 JAN)
      The fusion of transport vesicles with their target membranes is fundamental for intracellular membrane trafficking and diverse physiological processes and is driven by the assembly of functional soluble N-ethylmaleimide-sensitive ...
    • The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport 

      Laufman, Orly; Hong, WanJin; Lev, Sima; 洪万进 (J CELL BIOL, 2011-08-08)
      The conserved oligomeric Golgi (COG) complex has been implicated in the regulation of endosome to trans-Golgi network (TGN) retrograde trafficking in both yeast and mammals. However, the exact mechanisms by which it regulates ...
    • The deubiquitinase USP6 affects memory and synaptic plasticity through modulating NMDA receptor stability 

      Fanwei Zeng; 曾凡伟; Xuehai Ma; 马学海; Lin Zhu; 朱琳; Qiang Xu; Yuzhe Zeng; Yue Gao; Guilin Li; Tiantian Guo; Haibin Zhang; Xiaoyan Tang; Ziqiang Wang; Zesen Ye; Liangkai Zheng; Hongfeng Zhang; Qiuyang Zheng; Kunping Li; Jinfang Lu; Xueting Qi; Hong Luo; Xian Zhang; Zhanxiang Wang; Yulin Zhou; Yi Yao; Rongqin Ke; Ying Zhou; Ying Zhou; Hao Sun; Timothy Huang; Zhicheng Shao; Huaxi Xu; Xin Wang; 王鑫 (Public Library of Science, 2019-12-16)
      Ubiquitin-specific protease (USP) 6 is a hominoid deubiquitinating enzyme previously implicated in intellectual disability and autism spectrum disorder. Although these findings link USP6 to higher brain function, potential ...
    • UPLC-MS/MS法测定人血浆中金刚烷胺的含量 

      江峰 (2018-10-15)
      目的建立快速、灵敏、操作简便的UPLC-MS/MS法测定人血浆中金刚烷胺浓度。方法血浆经二氯甲烷提取,采用Acquity UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱,0.01%甲酸水(A)—甲醇(B)为流动相,流速0.25 ml·min-1,柱温30℃,应用电喷雾电离源,以多反应离子监测(MRM)方式进行检测,选择监测的离子为(m/z)152.2→135.48(金刚烷胺)和(m/z)166.29→148.29 ...
    • Viral-Host Interactions That Control HIV-1 Transcriptional Elongation 

      Huasong Lu; 路华松; Zichong Li; Yuhua Xue; Qiang Zhou; 周强 (American Chemical Society, 2013-06-24)
      Regulation of the pause and elongation by ribonucleic acid (RNA) polymerase II (Pol II) is used widely by metazoans to attain the pattern of gene expression that is essential for optimal cell growth/renewal, differentiation, ...
    • Z-十八碳-9-烯-丙磺酰胺对糖尿病认知功能障碍小鼠学习记忆功能的影响 

      王翠宝; 李莹; 卢华辉; 魏南服; 黄硕; 王韵; 饶荣; 许淑珍; 任桐 (2018-08-16)
      探索新化合物Z-十八碳-9-烯-丙磺酰胺(N15)对糖尿病认知功能障碍(DACD)小鼠学习记忆功能的作用及机制。采用链脲佐菌素(STZ)连续小剂量腹腔注射以及高脂高糖加STZ诱导2型糖尿病小鼠模型,N15(50和100mg·kg-1·d-1)连续灌胃给药6周,于给药末期进行跳台、避暗以及Morris水迷宫测试以评价小鼠学习记忆功能;对海马内葡萄糖和乳酸水平进行测定;通过realtimePCR测定海马内突触成长相关蛋白-43(GAP-43 ...
    • β内酰胺类在铜绿假单胞菌血流感染中的方案研究 

      李春华; 钱玉兰; 于迪; 李彦萍; 杭永付 (2017)
      目的制定某院B内酰胺类在铜绿假单胞菌(PA)血流感染中的治疗方案。方法收集某院2015年至2016年血培养中铜绿假单胞菌共64株,测定B内酰胺类(哌拉西林/他唑巴坦,头孢他啶,头孢吡肟,亚胺培南,美罗培南)对铜绿假单胞菌的最低抑菌浓度(MIC)。用蒙特卡洛模拟计算多种治疗方案的达标率(PrA)和累积反应分数(CFR)。结果经验治疗时,哌拉西彬他唑巴坦4.5g(q6h),头孢他啶2g(q8h),头孢吡肟2g(q8h),亚胺培南1g(q6h ...
    • 一个台湾变节者的“革命”前史——蔡孝乾早期历史探微 

      陈小冲 (2017)
      土地革命战争时期,中央苏区迎来了一批“台湾代表”,其中的主要人物蔡孝乾参与了中央苏区反帝总同盟的创立活动,并在之后的苏区反帝运动中发挥了一定的作用。蔡孝乾之得赴中央苏区,是中共因应大力开展反帝斗争及红五月运动的需要而主动运作的结果,其策划者为时任苏区中央局组织部长任弼时,中共苏区中央局书记周恩来应也给予了指导。然观察蔡孝乾早期历史可以发现,缺乏坚定革命信仰和工作中的被动性,是他最大的特点或弱点,此或为其日后变节行为埋下些许伏笔。
    • 一例重症肺炎合并戒酒综合征患者的病例分析 

      宁俊凯; 魏吟秋; 庞素秋 (2018-02-15)
      <正>由感染所致的肺炎最为常见,不同感染途径及不同宿主的肺炎在病原学上又具有不同的分布规律,现在对于肺炎的诊断多主张按照发病场所和宿主状态来分类,如社区获得性肺炎、医院获得(相关)性肺炎、老年人肺炎等,因此不同的肺炎其经验性抗感染方案也不尽相同。而抗感染药物作为药
    • “一带一路”视域下两岸文创产业合作探讨 

      石正方; 李嘉欣 (2018-12-20)
      \"一带一路\"建设重在\"民心互通\",而\"民心互通\"重在文化认同。推进中华文化与沿线地区多元文化交流互鉴从而构筑文化认同之基,需要文化自信为支撑。鉴于此,应从增强文化自信出发,坚持以提升中华文化凝聚力、增进中华文化生产力、扩大中华文化影响力为发展导向,立足海内外市场,依靠政府、企业及民间力量的协力合作,深化两岸文创产业合作。
    • 一组利尿合剂的配伍稳定性分析 

      黄艳 (2017)
      目的 通过实验讨论分析我院常用利尿合剂的配置稳定性以实现安全用药。方法 标准配置方法配置利尿合剂,测定混合药液及各成分的p H值,模拟不同配送和使用条件和不同配置顺序,定时记录温度和观察其澄明度。结果 室温不同光线的条件下,利尿合剂均为配置后7.5 h产生浑浊;冷藏的利尿合剂经过24 h仍为澄明液体,室外高温下配置后0.5 h即出现浑浊。在氯化钠注射液中先加入氨茶碱注射液而最后加入罂粟碱注射液的顺序配置的合剂稳定时间最长。结论 ...
    • 丁丙诺啡透皮贴剂治疗中重度慢性疼痛的疗效及安全性 

      柯金珍; 颜志文; 陈丰庆; 罗琪 (2017)
      目的观察丁丙诺啡透皮贴剂治疗中重度慢性疼痛的疗效和安全性。方法收集2015年10月至2016年3月在我院骨科门诊确诊为中重度慢性疼痛患者68例,给予丁丙诺啡透皮贴剂进行止痛治疗,初始剂量5 mg,qw,根据疼痛情况调整剂量至无痛或基本无痛状态。对每位患者随访1个月,分别评估患者治疗前后疼痛强度、镇痛效果、生活质量、用药依从性,同时监测药物不良反应。结果治疗前、后患者疼痛数字评分分别为(5.90±1.05)分和(1.35±0.75)分,P ...
    • 三叶青三氯甲烷部位化学成分及其抗肿瘤活性研究 

      丁丽; 章璐幸; 邱彦; 王延辉 (2015-11-8)
      目的研究三叶青三氯甲烷部位的化学成分及其体外抗肿瘤活性。方法利用硅胶,制备薄层板,lH-20等各种色谱技术分离单体化合物,通过nMr和质谱对化合物进行结构鉴定,并采用气-质联用色谱对三叶青三氯甲烷部位的其他成分进行分析,采用MTT法对分离得到的单体化合物进行抗肿瘤活性评价。结果通过经典色谱法分离得到6个化合物,分析鉴定为β-谷甾醇(1),4-羟基-3-甲氧基苯甲醛(2),齐墩果酸(3),5-羟甲基糠醛(4),壬二酸(5)和香草酸(6), ...