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dc.contributor.author罗雯
dc.contributor.author万雅各
dc.contributor.author彭宣宪
dc.contributor.author王三英
dc.date.accessioned2017-11-14T08:07:49Z
dc.date.available2017-11-14T08:07:49Z
dc.date.issued2001-11-30
dc.identifier.citation中华微生物学和免疫学杂志,2001,(06):107-109
dc.identifier.issn0254-5101
dc.identifier.otherZHWS200106037
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/159591
dc.description.abstract目的 建立一种快速检测病原菌的方法以利于临床诊断。方法 选取 10种具有代表性的病原菌 ,采用通用引物PCR(UPPCR)对其 16SrRNA基因进行扩增 ,并对PCR产物进行限制性酶切片段长度多态性分析 (RFLP)和单链构象多态性分析 (SSCP)。结果 RFLP电泳图谱呈现多态性 ,但半数菌的图谱两两相同或相似 ;SSCP电泳图谱各异 ,可以相互区分。结论 UPPCR SSCP技术能快速简便地检测病原菌
dc.description.abstractObjective To establish a method for the identification of pathogens for fast diagnosis. Methods By universal primer polymerase chain reaction (UPPCR), the 16S ribosomal RNA genes of ten representative pathogens were amplified. Then the PCR products were analyzed by single strand configuration polymorphism (SSCP) and restrict fragment length polymorphism (RFLP). Results RFLP map expressed polymorphism, but there were either the same or similar figures between two or among three pathogens in half of test strains. However, there were significantly different bands in all of the pathogens by SSCP. Conclusion UPPCR SSCP analysis can rapidly and conveniently identify bacterial pathogens. [
dc.description.sponsorship国家自然科学基金资助项目 ( 39770 5 85 );; IFS基金资助项目 (A 2 338 2)
dc.language.isozh_CN
dc.subject病原菌
dc.subject聚合酶链反应
dc.subject限制性酶切片段长度多态性
dc.subject单链构象多态性
dc.subjectPathogens
dc.subjectPCR
dc.subjectRFLP
dc.subjectSSCP
dc.title采用通用引物PCR配合SSCP及RFLP技术快速检测常见病原菌
dc.title.alternativeRapid identification of pathogens using UPPCR with SSCP and RFLP analysis
dc.typeArticle


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