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dc.contributor.authorLi, Xuemin
dc.contributor.authorChen, Mingmao
dc.contributor.authorYang, Wenzhi
dc.contributor.authorZhou, Zhimin
dc.contributor.authorLiu, Lingrong
dc.contributor.authorZhang, Qiqing
dc.contributor.author张其清
dc.date.accessioned2013-04-18T06:43:14Z
dc.date.available2013-04-18T06:43:14Z
dc.date.issued2012-04
dc.identifier.citationCOLLOIDS AND SURFACES B-BIOINTERFACES,2012,92:136-141zh_CN
dc.identifier.issn0927-7765
dc.identifier.urihttp://dx.doi.org/10.1016/j.colsurfb.2011.11.030
dc.identifier.uriWOS:000300859000019
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15733
dc.description.abstractIn order to understand the nanomedicine and nanotoxicological effects of self-assembled nanoparticles of 6-O-cholesterol modified chitosan (O-CHCS NPs) as a carrier for drug delivery systems, the interaction between O-CHCS NPs and bovine serum albumin (BSA) was studied by spectroscopy and calorimetric methods. The morphology of the complex between O-CHCS NPs and BSA observed by transmission electron microscope (TEM) was almost spherical shape. The size and the zeta potential of the complex increased with the concentration of O-CHCS NPs increasing. The fluorescence spectroscopy indicated that the micro-environment around the tryptophan (Trp) residues in BSA had slight change due to only partially exposure of the Trp residues to water in the interaction process. Compared with free BSA, the addition of O-CHCS NPs led to the decrease of alpha-helical content of BSA and the increase of beta-strand content. Isothermal titration calorimeter (ITC) results showed that the binding reaction between O-CHCS NPs and BSA was exothermic and enthalpically driven. Therefore, it could be concluded that hydrogen bonding, hydrophobic and electrostatic interactions played a key role in the complex formation, and the formation mechanism was proposed accordingly. In addition, cytotoxicity assay implied that O-CHCS NPs were non-cytotoxic and biocompatible up to 200 mu g mL(-1). These data demonstrated the potential application of O-CHCS NPs for drug delivery. (C) 2011 Elsevier B.V. All rights reserved.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [90923042]; Ministry of Education of China [20101106110042]; National Key Scientific Projects of China [2006CB933300]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subject6-O-Cholesterol modified chitosanzh_CN
dc.subjectBovine serum albuminzh_CN
dc.subjectNanoparticlezh_CN
dc.subjectInteractionzh_CN
dc.subjectPolymeric amphiphilezh_CN
dc.titleInteraction of bovine serum albumin with self-assembled nanoparticles of 6-O-cholesterol modified chitosanzh_CN
dc.typeArticlezh_CN


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