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dc.contributor.authorHu, Su Xian
dc.contributor.authorSui, Hua Xiu
dc.contributor.authorJin, Hao Jie
dc.contributor.authorNi, Xiao Yan
dc.contributor.authorLiu, Xiao Xing
dc.contributor.authorXue, Mao Qiang
dc.contributor.authorZhang, Ye
dc.contributor.authorGao, Feng Guang
dc.contributor.author高丰光
dc.date.accessioned2013-04-18T01:31:03Z
dc.date.available2013-04-18T01:31:03Z
dc.date.issued2012-04
dc.identifier.citationMOLECULAR MEDICINE REPORTS,2012,5(4);1005-1010zh_CN
dc.identifier.issn1791-2997
dc.identifier.urihttp://dx.doi.org/10.3892/mmr.2012.751
dc.identifier.uriWOS:000301324100022
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15726
dc.description.abstractThe reported effects of nicotine on dendritic cells (DCs) are controversial. To investigate the factors which determine the effects of nicotine on DCs, immature dendritic cells (imDCs) induced from murine bone marrow were treated with different doses of nicotine with or without lipopolysaccharides (LPS). The morphology and expression of the co-stimulatory molecules CD80, CD86, CD40 and CD54 were observed and determined by microscopy and flow cytometry, respectively. The results showed that, firstly, nicotine treatment promoted the development of DC precursors into imDCs with a semi-mature phenotype revealed by a higher expression of CD11c and more branched projections. Secondly, lower doses of nicotine (16.5 ng/ml), but not higher (200 mu g/ml), up-regulated the expression of the co-stimulatory molecules CD80, CD40 and CD54 on imDCs. Co-administration of LPS and nicotine revealed differential effects on co-stimulatory molecule expression on imDCs. Thirdly and importantly, treatment with lower doses of nicotine (16.5 ng/ml) did not augment expression of the CD80, CD86, CD40 and CD54 molecules in mature DCs. Fourthly and interestingly, high doses of nicotine (more than 165 mu g/ml) revealed pro-apoptotic activity but lower doses of nicotine (16.5-0.165 ng/ml) achieved an antiapoptotic effect on imDCs. All data presented here indicate that the controversial effects of nicotine on DCs may be due to the LPS of the nicotinic environment and the dose of nicotine used.zh_CN
dc.description.sponsorshipNatural Science Foundation of Fujian Province of China [2008J0112]; Natural Science Foundation of Xiamen [3502Z20104002]; Xiamen Science and Technology Key program [3502Z20100006]zh_CN
dc.language.isoenzh_CN
dc.publisherSPANDIDOS PUBL LTDzh_CN
dc.subjectnicotinezh_CN
dc.subjectdendritic cellszh_CN
dc.subjectlipopolysaccharideszh_CN
dc.subjectco-stimulatory moleculeszh_CN
dc.titleLipopolysaccharide and dose of nicotine determine the effects of nicotine on murine bone marrow-derived dendritic cellszh_CN
dc.typeArticlezh_CN


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