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dc.contributor.author陈美灵
dc.contributor.author于金金
dc.contributor.author吴晓琛
dc.contributor.author魏冬梅(台州学院生命科学学院)
dc.contributor.author田惠桥
dc.date.accessioned2013-04-12T02:26:55Z
dc.date.available2013-04-12T02:26:55Z
dc.date.issued2012-11
dc.identifier.citation中国细胞生物学学报,2012, 34(11):1123–1128zh_CN
dc.identifier.issn0253-9977
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15588
dc.description通讯作者。Tel: 0592-2186486, E-mail: hqtian@xmu.edu.cnzh_CN
dc.description.abstract[中文摘要]将洋葱的胚珠置于酶液中酶解50~110 min后剥去其珠被,可清楚地看到珠心中的胚囊轮廓。用解剖针将珠心从中部横切,然后挤压其珠孔部位,卵器细胞从胚珠的切口处逸出。再用显微操作仪的玻璃针将卵细胞和两个助细胞分开,达到分离洋葱卵细胞的目的。酶对分离卵细胞具有重要作用,在最佳的酶液浓度[0.02%果胶酶Y23、0.08%果胶酶(Serva)、0.05%纤维素酶和0.05%半纤维素酶]下酶解胚珠110 min后,解剖1 h可从24个胚珠中分离出10个卵细胞(41.67%)。随着胚囊的发育,两个助细胞的体积出现明显的二形性。洋葱生活卵细胞的分离为开展洋葱离体受精建立了基础,也为研究洋葱卵器细胞的发育创造了条件。[英文摘要]Viable egg cells of Allium cepa L.were isolated using enzymic digestion and mechanical dissection.The onion ovules were incubated in an enzymic solution for 50~110 min and then peeled its two intaguments.The outline of embryo sac in nucellus was seen clearly.Using a dissecting needle cutting necellus transversely and pushing its micropyle part,three cells of egg apparatus could be released from cut end of the nucellus.The egg cell could be separated with two synergids,reaching the purpose of isolation of onion egg cell. Enzymes are very important for isolating egg cell, and in optimal enzyme solution containing 0.02% Pectolyse Y-23, 0.08% Pectinase (Serva), 0.05% Cellulase (Onozuka RS), 0.05% Hemicellulase (Sigma), 10 egg cells (41.67%) could be isolated from 24 ovules in 1 h. With embryo sac development, two synergids display dimorphism in size. The isolation of onion egg cells makes a base for its in vitro fertilization, and provides the condition to study onion egg development using methods of molecular biology.zh_CN
dc.description.sponsorship国家自然科学基金(No.31170289); 公益性行业(农业)科研专项经费(No.200903016); 浙江省自然科学基金(No.Y3110395)资助项目.zh_CN
dc.language.isozhzh_CN
dc.publisher《中国细胞生物学学报》编辑部zh_CN
dc.subject洋葱zh_CN
dc.subject卵细胞zh_CN
dc.subject分离zh_CN
dc.subjectAllium cepazh_CN
dc.subjectegg cellzh_CN
dc.subjectisolationzh_CN
dc.title洋葱卵细胞的分离zh_CN
dc.title.alternativeIsolation of Egg Cells from Allium cepa L.zh_CN
dc.typeArticlezh_CN


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