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dc.contributor.authorWang, Jinglei
dc.contributor.authorHuang, Yong
dc.contributor.authorZou, Quan
dc.contributor.author邹权
dc.contributor.authorShen, Xing Jia
dc.contributor.authorYu, Xue Li
dc.contributor.authorWang, Zhan Bin
dc.contributor.authorCheng, Xiang Chao
dc.date.accessioned2013-04-11T08:42:33Z
dc.date.available2013-04-11T08:42:33Z
dc.date.issued2012-04
dc.identifier.citationMOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY,2012,27(2):85-90zh_CN
dc.identifier.issn0891-4168
dc.identifier.urihttp://dx.doi.org/10.3103/S0891416812020036
dc.identifier.uriWOS:000308291400007
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15579
dc.description.abstractMicroRNAs (miRNAs) are endogenous small non-protein coding RNAs that play important regulatory roles in animals and plants by binding to target transcripts for cleavage or translational repression. The miR-9a is very conservative in animals from flies to humans. Studies indicated that miR-9a is involved in the regulation of neurogenesis in animals. In our study, the baculovirus expression system was used to transcribe a recombinant vector containing miR-9a for further analysis the function of miR-9a. The sequence of premiR-9a from silkworm DNA was first cloned into the donor pFastBac. The enhanced green fluorescent protein (EGFP) was used as reporter gene. The recombinant donor plasmid pFastBac-miR-9a was transformed into E. coli DH10Bac/AcNPV forming bacmid-9a which was transfected into insect cells with cational lipofectin. The transcription of mature miR-9a was detected by Real-time PCR. The results show the recombinant bacmid-9a was successfully constructed and effectively transcribed miR-9a in ipfected Sf21 insect cells.zh_CN
dc.description.sponsorshipDoctoral Science Foundation of He Nan University of Science [09001578]; Natural Science Foundation of China [31172266]zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGERzh_CN
dc.subjectbaculovirus expression systemzh_CN
dc.subjectmiR-9azh_CN
dc.subjectAcNPVzh_CN
dc.subjectSf21 cellszh_CN
dc.titleConstruction of baculovirus expression vector of miRNAs and its expression in insect cellszh_CN
dc.typeArticlezh_CN


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