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dc.contributor.authorZhang, Yiru
dc.contributor.author张毅汝
dc.contributor.authorXing, Yuqian
dc.contributor.authorZhang, Lei
dc.contributor.authorMei, Yang
dc.contributor.authorYamamoto, Kazuo
dc.contributor.authorMak, Tak W.
dc.contributor.authorYou, Han
dc.date.accessioned2013-04-11T03:27:28Z
dc.date.available2013-04-11T03:27:28Z
dc.date.issued2012-04-10
dc.identifier.citationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(15):5717-5722zh_CN
dc.identifier.issn0027-8424
dc.identifier.urihttp://dx.doi.org/10.1073/pnas.1203210109
dc.identifier.uriWOS:000302533500037
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15573
dc.description.abstractTo ensure genome stability, DNA must be replicated once and only once during each cell cycle. Cdt1 is tightly regulated to make sure that cells do not rereplicate their DNA. Multiple regulatory mechanisms operate to ensure degradation of Cdt1 in S phase. However, little is known about the positive regulators of Cdt1 under physiological conditions. Here we identify FOXO3 as a binding partner of Cdt1. FOXO3 forms a protein complex with Cdt1, which in turn blocks its interaction with DDB1 and PCNA. Conversely, FOXO3 depletion facilitated the proteolysis of Cdt1 in unperturbed cells. Intriguingly, FOXO3 deficiency resulted in impaired S-phase entry and reduced cell proliferation. We provide data that FOXO3 knockdown mimics Cdt1 down-regulation and affects G1/S transitions. Our results demonstrate a unique role of FOXO3 in binding to Cdt1 and maintaining its level required for cell cycle progression.zh_CN
dc.description.sponsorshipChina-Canada Collaborative Research National Natural Science Foundation of China [30700130]; Canadian Institutes of Health Research; National Basic Research Program of China 973 Program [2009CB522202]; Ministry of Education of China [B06016]zh_CN
dc.language.isoenzh_CN
dc.publisherNATL ACAD SCIENCESzh_CN
dc.subjectUBIQUITIN LIGASE COMPLEXzh_CN
dc.subjectGEMININ BINDINGzh_CN
dc.subjectNUCLEAR ANTIGENzh_CN
dc.subjectS-PHASEzh_CN
dc.subjectDEGRADATIONzh_CN
dc.subjectDAMAGEzh_CN
dc.subjectPROTEOLYSISzh_CN
dc.subjectINHIBITIONzh_CN
dc.subjectPROTEINSzh_CN
dc.subjectPCNAzh_CN
dc.titleRegulation of cell cycle progression by forkhead transcription factor FOXO3 through its binding partner DNA replication factor Cdt1zh_CN
dc.typeArticlezh_CN


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