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dc.contributor.authorDeng, Hua
dc.contributor.authorWang, Ya-Ying
dc.contributor.authorZhu, Xue-Yi
dc.contributor.author朱学艺
dc.contributor.authorTian, Hui-Qiao
dc.contributor.author田惠桥
dc.date.accessioned2013-04-09T03:29:36Z
dc.date.available2013-04-09T03:29:36Z
dc.date.issued2012-04-26
dc.identifier.citationANNALES BOTANICI FENNICI,2012,49:1-6zh_CN
dc.identifier.issn0003-3847
dc.identifier.uriWOS:000303272200001
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/15546
dc.description.abstractBauhinia blakeana (Fabaceae) pollen is bicellular and contains only a vegetative and generative cell at anthesis. The pollen grain contents, including the generative cell, can be released in vitro following osmotic shock. In this study, when pollen grains were incubated in a 10% sucrose and 5%-15% polyethylene glycol 4500 (PEG 4500) medium, over 50% of the pollen grains germinated and formed a short pollen tube. However, the generative cell did not divide in in vitro pollen tubes. In vivo pollen tubes were induced to grow from the style using an in vivo-in vitro method. Sperm cells were subsequently isolated from broken pollen tubes.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China [31170289]; Special Fund for Agro-scientific Research in the Public Interest of Agricultural department of China [200903016]zh_CN
dc.language.isoenzh_CN
dc.publisherFINNISH ZOOLOGICAL BOTANICAL PUBLISHING BOARDzh_CN
dc.subjectPOLLEN TUBESzh_CN
dc.subjectFERTILIZATIONzh_CN
dc.titleGenerative and sperm cell isolation in Bauhinia blakeana (Fabaceae)zh_CN
dc.typeArticlezh_CN


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