Sensitivity of Thallus Cells of Porphyra haitanensis to Four Organic Solvent
- 生命科学－已发表论文 
分析了坛紫菜叶状体细胞对甲醇、乙醇、异丙醇、1, 3 丙二醇等4种有机溶剂的敏感性.结果表明:体细胞对1, 3 丙二醇敏感,在实验最低剂量(0. 2μL/mL)下对紫菜体细胞就表现出较强的致死作用;对甲醇有较强的耐受性,在最高的1. 0μL/mL处理浓度组,生长发育状况与对照组无明显差别;乙醇在浓度高于0. 6μL/mL时强烈抑制体细胞的分裂;异丙醇随着浓度的升高表现出使体细胞向细胞团方向分化,而叶状体的形成则受到抑制.本实验结果表明,在用外源化合物处理紫菜叶状体细胞时,甲醇在1. 0μL/mL浓度范围内可作为合适的有机溶剂.The paper reported sensitivity test of thallus cells of Porphyra haitanensis to four common used organic solvents,methanol,ethanol,isopropanol,and 1,3-propanediol.Protoplasts of Porphyra haitanensis were prepared by sea snail enzyme digestion,and treated with organic solvents which the concentration gradient was 0.2 μL/mL,0.4 μL/mL,0.6 μL/mL,0.8 μL/mL,1.0 μL/mL dissolved in culture solution.The morphological differentiation of the cells treated with different organic solvents was observed with microscope and the survival rate of thallus cells was sampled every three days for 15 days.The data were analyzed with “SPSS 11.0” program.The result showed that in the lowest dose (0.2 μL/mL),1,3-propylene glycol was lethal to the thallus cells;however,the thallus cells showed intensively tolerant to methanol even in the highest dose (1.0 μL/mL).It was shown that division of somatic cell was obviously inhibited by ethanol when the concentration was 0.6 μL/mL.Differentiation of somatic cell was restrained more strongly with raising dose of isopropanol.It is suggested that methanol can be the appropriate organic solvent in the dose less than (1.0) μL/mL when thallus cells of Porphyra haitanensis were treated with chemical substances such as antibiotics or phytohormone.