Inhibitory effects of tachyplesin on the proliferation of human hepatocarcinoma cell line SMMC-7721
- 生命科学－已发表论文 
应用从中国鲎血细胞中提取的鲎素处理人肝癌SMMC-7721细胞,研究海洋生物活性物质的抗肿瘤作用。实验结果显示,经3.0mg·L-1鲎素处理的SMMC-7721细胞生长缓慢,细胞生长抑制率为55.57%,细胞分裂指数抑制率达43.68%;处理后的细胞碱性磷酸酶活性降低36.26%,γ-GT活性下降,TAT活性增强。结果表明,鲎素能有效地抑制肝癌细胞的增殖活动,具有与癌细胞诱导分化物相似的抗肿瘤效果。Tachyplesin, a marine bioactive substance which was isolated from acid extracts of horseshoe crab (Tachypleus tridentatus) hemocytes, was used to investigate the effects of tachyplesin on the proliferation of human hepatocarcinoma cell line SMMC-7721. The trypan excluision and Hematoxylin-Eosin dyeing were applied to examine the growth curve and mi-totic index respectively. Biochemistry was used to assay the activities of alkaline phos-phatase, γ-GT and TAT. The results showed that the proliferation of SMMC-7721 cells was inhibited obviously after treating with 3.0ug/ml tachyplesin, the growth inhibitory rate amounted to 55. 57%, and the cell mitotic index decreased by 43. 68%. In the meantime, tachyplesin could decrease the activities of alkaline phosphatase and γ-GT, while increase the activity of TAT. It was demonstrated that tachyplesin could efectively inhibit the proliferation of the human hepatocarcinoma SMMC-7721 cell and induce the cell differentiation.