The Noninvasive DNA Extraction and Detection of Silkworms
- 生命科学－已发表论文 
对家蚕和蓖麻蚕的蚕蜕和蛾翅分别采用SDS裂解法和动物组织基因组试剂盒提取法提取DNA ,并与常规方法以蚕蛹提取的DNA一起进行RAPD扩增图谱比较、分析 ,结果表明用非损伤性方法提取的DNA的RAPD图谱和用常规方法提取的DNA的RAPD图谱基本一致To obtain DNA noninvasively from the silkworms Bombyx mori and Philosamia Cynthia ricini, we collected the sloughs of fifth instar and the wings of moth, and then extracted DNA by SDS and the DNA extraction kit of animal tissue and purified them. These DNA samples were analyzed by RAPD with 8 arbitrary primes. The results indicated that all the DNA samples had good quality, and that the RAPD pattern of the DNA samples from the silkworms Bombyx mori and Philosamia Cynthia ricini were respectively almost identical.