Studying the growth metabolism of Aeromonas hydrophila with the methods of proteomics
- 生命科学－已发表论文 
采用蛋白质组学的方法之一二维电泳技术 ,对嗜水气单胞菌生长周期中各不同阶段的蛋白质组成进行了分析。结果显示 ,在不同生长阶段 ,其蛋白质组成均发生明显变化。发现了与细菌生长代谢各阶段生理活动有关的差异蛋白质 ,有助于深入了解嗜水气单胞菌生长周期中不同阶段的基因组表达情况。同时 ,还对二维电泳和普通SDS 聚丙烯酰胺凝胶电泳进行比较。结果表明 ,二维电泳技术是研究蛋白质组学的有效工具Aeromonas hydrophila is one of the important pathogenic bacteria of aquaculture. It is significant, therefore, to study the changes on functional genome in its growth cycle. Two dimensional electrophoresis (2 D PAGE), one of the approaches for proteomics, was used to study protein composition in different phases during the cycle. The result showed that the composition was significantly changed in different phases. The discovery of different pertinent proteins in the different growth phases will contribute to find out the genome expression of A. hydrophila in different growth phases. In this experiment 2 D PAGE was compared with normal SDS PAGE in the same conditions, and the result indicated that 2 D PAGE was an effective technique for proteomics study.