Analysis of Protein and Genomic DNA of Echinochasmus fujianensis
- 生命科学－已发表论文 
采用RAPD技术和等电聚焦电泳方法 ,对福建棘隙吸虫福建株和广东株虫体进行基因组DNA多态性分析和蛋白质等电点测定 .结果表明 ,15个随机引物在两虫株基因组DNA中共获得扩增带 182条 ,二者共有的 89条 ,广东株特有的 4条 ,其共享度为 0 .978,遗传距离指数为 0 .0 2 2 ;两虫株的蛋白质经电泳分离均可获得 9条区带 ,其中有 7条区带的等电点相同 ,第Ⅳ、Ⅴ条蛋白区带的等电点存在差异 .研究表明在福建和广东两地流行的福建棘隙吸虫是同一种属By using RAPD technique and isoelectric focusing electrophoresis method, genomic DNA and protein from Guangdong strain and Fujian strain of Echinochasmus fujianensis were analysed respectively. One hundred and eighty-two polymorphic DNA fragments were generated by RAPD with fifteen primers. Among them, eighty-nine fragments were shared by two strains. Four fragments in Guangdong strain amplified by three primers were absent from Fujian strain. Their haplotype diversity indices and genetic distance were 0.978 and 0.022 respectively. The result indicated that Guangdong strain and Fujian strain were in the same genotype. There were nine protein bands generated by isoelectric focusing electrophoresis in these E. fujianensis. Their protein characters were acidic. Their isoelectric points were in the range of 4.3～6.4. With the exception of two band, all of the bands had neither significant isoelectric point diversity nor visible relative content diversity between E. fujianensis Guangdong strain and Fujian strain. The result indicated that E. fujianensis Guangdong strain and Fujian strain were the same species.